Anti-DDX55 Antibody

DDX55 antibody

Boster Bio Anti-DDX55 Antibody (Catalog # A12850). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A12850
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-DDX55 Antibody

View all DDX55 Antibodies

SKU/Catalog Number

A12850

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-DDX55 Antibody (Catalog # A12850). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DDX55 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12850)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human DDX55.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12850 is reactive to DDX55 in Human, Mouse

Applications

A12850 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

68547 MW

Background of DDX55

Probable ATP-binding RNA helicase.

The MGC Project Team; Genome Res. 14:2121-2127(2004).
The German cDNA consortium; Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
Nagase T., DNA Res. 7:273-281(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For DDX55 (Source: Uniprot.org, NCBI)

Gene Name

DDX55

Full Name

ATP-dependent RNA helicase DDX55

Weight

68547 MW

Superfamily

DEAD box helicase family

Alternative Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 55; DEAD box protein 55; EC 3.6.1; EC 3.6.4.13; FLJ16577; KIAA1595ATP-dependent RNA helicase DDX55; MGC33209 DDX55 DEAD-box helicase 55 ATP-dependent RNA helicase DDX55|DEAD (Asp-Glu-Ala-Asp) box polypeptide 55|DEAD box protein 55|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DDX55, check out the DDX55 Infographic

DDX55 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DDX55: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-DDX55 Antibody

Question

Does A12850 anti-DDX55 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

It shows on the product datasheet, A12850 anti-DDX55 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-22

Question

We are currently using anti-DDX55 antibody A12850 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

The anti-DDX55 antibody (A12850) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-16

Question

Is this A12850 anti-DDX55 antibody reactive to the isotypes of DDX55?

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

The immunogen of A12850 anti-DDX55 antibody is Synthesized peptide derived from internal of human DDX55. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-05

Question

I was wanting to use your anti-DDX55 antibody for WB for mouse cerebellar hemisphere on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cerebellar hemisphere identification?

A. Johnson

Verified customer

Asked: 2017-05-03

Answer

You can see on the product datasheet, A12850 anti-DDX55 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cerebellar hemisphere in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-05-03

Question

Does anti-DDX55 antibody A12850 work for WB with cerebellar hemisphere?

E. Krishna

Verified customer

Asked: 2015-10-27

Answer

According to the expression profile of cerebellar hemisphere, DDX55 is highly expressed in cerebellar hemisphere. So, it is likely that anti-DDX55 antibody A12850 will work for WB with cerebellar hemisphere.

Boster Scientific Support

Answered: 2015-10-27

Order DetailsPrice
A12850-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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