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Product Info Summary
| SKU: | A03288 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PHF8 Monoclonal Antibody
SKU/Catalog Number
A03288
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PHF8 Monoclonal Antibody catalog # A03288. Tested in WB applications. This antibody reacts with Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHF8 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03288)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Isotype
IgG
Reactive Species
A03288 is reactive to PHF8 in Human, Mouse, Pig, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
117864 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03288 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis using PHF8 Monoclonal antibody against A451,293 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PHF8 (Source: Uniprot.org, NCBI)
Gene Name
PHF8
Full Name
Histone lysine demethylase PHF8
Weight
117864 MW
Superfamily
JHDM1 histone demethylase family
Alternative Names
Histone lysine demethylase PHF8;1.14.11.27;PHD finger protein 8;PHF8;KIAA1111, ZNF422; PHF8 JHDM1F, KDM7B, MRXSSD, ZNF422 PHD finger protein 8 histone lysine demethylase PHF8|[histone H3]-dimethyl-L-lysine(36) demethylase PHF8|[histone H3]-dimethyl-L-lysine(9) demethylase PHF8|jumonji C domain-containing histone demethylase 1F
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHF8, check out the PHF8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHF8 Monoclonal Antibody (A03288)
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No publications found for A03288
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Customer Q&As
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5 Customer Q&As for Anti-PHF8 Monoclonal Antibody
Question
Is this A03288 anti-PHF8 Monoclonal antibody reactive to the isotypes of PHF8?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The immunogen of A03288 anti-PHF8 Monoclonal antibody is Purified recombinant human PHF8 (C-terminal) protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-10
Question
I was wanting to use your anti-PHF8 Monoclonal antibody for WB for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?
C. Miller
Verified customer
Asked: 2019-07-17
Answer
You can see on the product datasheet, A03288 anti-PHF8 Monoclonal antibody has been validated for WB on human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-17
Question
I have a question about product A03288, anti-PHF8 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Miller
Verified customer
Asked: 2019-05-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03288 anti-PHF8 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-22
Question
Will anti-PHF8 Monoclonal antibody A03288 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
According to the expression profile of cervix carcinoma erythroleukemia, PHF8 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-PHF8 Monoclonal antibody A03288 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-01-25
Question
Do you have a BSA free version of anti-PHF8 Monoclonal antibody A03288 available?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PHF8 Monoclonal antibody A03288 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-03
