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Product Info Summary
| SKU: | A03758-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PHF1 Antibody Picoband®
SKU/Catalog Number
A03758-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PHF1 Antibody Picoband® catalog # A03758-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PHF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03758-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PHF1 recombinant protein (Position: L31-H491).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03758-2 is reactive to PHF1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
62 kDa
Calculated molecular weight
62.106kDa
Background of PHF1
PHD finger protein 1 is a protein that in humans is encoded by the PHF1 gene. This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03758-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat heart tissue, mouse heart tissue, mouse C2C12 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PHF1 using anti-PHF1 antibody (A03758-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates,
Lane 3: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF1 antigen affinity purified polyclonal antibody (Catalog # A03758-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF1 at approximately 62 kDa. The expected band size for PHF1 is at 62 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PHF1 (Source: Uniprot.org, NCBI)
Gene Name
PHF1
Full Name
PHD finger protein 1
Weight
62.106kDa
Superfamily
Polycomblike family
Alternative Names
Sodium/glucose cotransporter 2; Na (+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2; SLC5A2; SGLT2 PHF1 MTF2L2, PCL1, PHF2, TDRD19C, hPHF1 PHD finger protein 1 PHD finger protein 1|hPCl1|polycomb-like 1|polycomb-like protein 1|testicular tissue protein Li 140|tudor domain containing 19C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHF1, check out the PHF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHF1 Antibody Picoband® (A03758-2)
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