Anti-PHF1 Antibody Picoband™

Product Name

Anti-PHF1 Antibody Picoband™

View all PHF1 Antibodies

SKU/Catalog Number

A03758-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PHF1 Antibody Picoband™ catalog # A03758-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PHF1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03758-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PHF1 recombinant protein (Position: L31-H491).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03758-2 is reactive to PHF1 in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

62 kDa

Calculated molecular weight

Background of PHF1

PHD finger protein 1 is a protein that in humans is encoded by the PHF1 gene. This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03758-2 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Positive Control

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PHF1 using anti-PHF1 antibody (A03758-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates,
Lane 3: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF1 antigen affinity purified polyclonal antibody (Catalog # A03758-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF1 at approximately 62 kDa. The expected band size for PHF1 is at 62 kDa.

Gene/Protein Information For PHF1 (Source: Uniprot.org, NCBI)

Alternative Names

hPCl1; MTF2L2; PCL1; PHD finger protein 1; PHF2; Polycomb-like protein 1; Protein PHF1 PHF1 MTF2L2, PCL1, PHF2, TDRD19C, hPHF1 PHD finger protein 1 PHD finger protein 1|hPCl1|polycomb-like 1|polycomb-like protein 1|testicular tissue protein Li 140|tudor domain containing 19C

For more info on PHF1, check out the PHF1 Infographic

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In this infographic, you will see the following information for PHF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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