Product Info Summary
SKU: | M01449-1 |
---|---|
Size: | 50 µl |
Reactive Species: | Human, Mouse |
Host: | Mouse |
Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-PGK1 Antibody
SKU/Catalog Number
M01449-1
Size
50 µl
Description
Boster Bio Anti-PGK1 Antibody (Catalog # M01449-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PGK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01449-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1086CT10.2.1
Isotype
IgG2a,k
Immunogen
This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-417 amino acids from human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01449-1 is reactive to PGK1 in Human, Mouse
Applications
M01449-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44615 Da
Background of PGK1
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:8000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
![m01449 1 anti pgk1 antibody wb testing 1 m01449 1 anti pgk1 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m01449-1-anti-pgk1-antibody-wb-testing-1.jpg)
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All lanes : Anti-PGK1 Antibody at 1:8000 dilution
Lane 1: 293 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 45 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
![m01449 1 anti pgk1 antibody ihc p testing 2 m01449 1 anti pgk1 antibody ihc p testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m01449-1-anti-pgk1-antibody-ihc-p-testing-2.jpg)
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M01449-1 staining PGK1 in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
![m01449 1 anti pgk1 antibody ihc p testing 3 m01449 1 anti pgk1 antibody ihc p testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m01449-1-anti-pgk1-antibody-ihc-p-testing-3.jpg)
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M01449-1 staining PGK1 in human liver tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
![m01449 1 anti pgk1 antibody fc testing 4 m01449 1 anti pgk1 antibody fc testing 4](https://www.bosterbio.com/media/catalog/product/m/0/m01449-1-anti-pgk1-antibody-fc-testing-4.jpg)
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Overlay histogram showing Jurkat cells stained with M01449-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M01449-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For PGK1 (Source: Uniprot.org, NCBI)
Gene Name
PGK1
Full Name
Phosphoglycerate kinase 1
Weight
44615 Da
Superfamily
phosphoglycerate kinase family
Alternative Names
Cell migration-inducing gene 10 protein; EC 2.7.2.3; MGC142128; MGC8947; MIG10; PGK1; PGKA; PGKAMGC117307; phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2; PRP2 PGK1 HEL-S-68p, MIG10, PGKA phosphoglycerate kinase 1 phosphoglycerate kinase 1|PRP 2|cell migration-inducing gene 10 protein|epididymis secretory sperm binding protein Li 68p|primer recognition protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PGK1, check out the PGK1 Infographic
![PGK1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PGK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PGK1 Antibody (M01449-1)
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