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Product Info Summary
| SKU: | A04716 |
|---|---|
| Size: | 100µg |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PGF2α Receptor (FP) Ptgfr Antibody
SKU/Catalog Number
A04716
Size
100µg
Form
Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Description
Boster Bio Anti-PGF2α Receptor (FP) Ptgfr Antibody catalog # A04716. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PGF2α Receptor (FP) Ptgfr Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04716)
Host
Rabbit
Contents
Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Synthetic peptide corresponding to the sequence near the N-terminus of rat PGF2 Receptor.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04716 is reactive to Ptgfr in Mouse, Rat
Reconstitution
Calculated molecular weight
90174 MW
Background of PTGFR
SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04716 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Membrane ELISA:Lewis rat striatum membranes (5µ
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Figure 1. Western blot analysis of Ptgfr using anti-Ptgfr antibody (A04716).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ptgfr antigen affinity purified polyclonal antibody (Catalog # A04716) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Ptgfr.
Protein Target Info & Infographic
Gene/Protein Information For Ptgfr (Source: Uniprot.org, NCBI)
Gene Name
Ptgfr
Full Name
Prostaglandin F2-alpha receptor
Weight
90174 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Dapper homolog 1;hDPR1;Dapper antagonist of catenin 1;Hepatocellular carcinoma novel gene 3 protein;DACT1;DPR1, HNG3; PTGFR FP prostaglandin F receptor prostaglandin F2-alpha receptor|FP prostanoid receptor|PGF receptor|PGF2 alpha receptor|prostaglandin receptor (2-alpha)|prostanoid FP receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ptgfr, check out the Ptgfr Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ptgfr: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PGF2α Receptor (FP) Ptgfr Antibody (A04716)
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No publications found for A04716
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Customer Q&As
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13 Customer Q&As for Anti-PGF2α Receptor (FP) Ptgfr Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-PGF2α Receptor (FP) antibody A04716. Let me know if you need anything else.
Z. Patel
Verified customer
Asked: 2020-04-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-30
Question
I see that the anti-PGF2α Receptor (FP) antibody A04716 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-19
Question
Is a blocking peptide available for product anti-PGF2α Receptor (FP) antibody (A04716)?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
We do provide the blocking peptide for product anti-PGF2α Receptor (FP) antibody (A04716). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-22
Question
Will anti-PGF2α Receptor (FP) antibody A04716 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
According to the expression profile of liver, PTGFR is highly expressed in liver. So, it is likely that anti-PGF2α Receptor (FP) antibody A04716 will work for WB with liver.
Boster Scientific Support
Answered: 2019-12-04
Question
We are currently using anti-PGF2α Receptor (FP) antibody A04716 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The anti-PGF2α Receptor (FP) antibody (A04716) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-12
Question
Would A04716 anti-PGF2α Receptor (FP) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
It shows on the product datasheet, A04716 anti-PGF2α Receptor (FP) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-21
Question
Is this A04716 anti-PGF2α Receptor (FP) antibody reactive to the isotypes of PTGFR?
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
The immunogen of A04716 anti-PGF2α Receptor (FP) antibody is Synthetic peptide corresponding to the sequence near the N-terminus of rat PGF2 Receptor. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-27
Question
Will anti-PGF2α Receptor (FP) antibody A04716 work on bovine WB with trachea?
T. Miller
Verified customer
Asked: 2018-01-04
Answer
Our lab technicians have not validated anti-PGF2α Receptor (FP) antibody A04716 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-PGF2α Receptor (FP) antibody A04716 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine trachea in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-04
Question
My question regarding product A04716, anti-PGF2α Receptor (FP) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04716 anti-PGF2α Receptor (FP) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-05
Question
I was wanting to use your anti-PGF2α Receptor (FP) antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
It shows on the product datasheet, A04716 anti-PGF2α Receptor (FP) antibody has been validated for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-11
Question
I am interested in to test anti-PGF2α Receptor (FP) antibody A04716 on rat liver for research purposes, then I may be interested in using anti-PGF2α Receptor (FP) antibody A04716 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
The products we sell, including anti-PGF2α Receptor (FP) antibody A04716, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-25
Question
Do you have a BSA free version of anti-PGF2α Receptor (FP) antibody A04716 available?
H. Collins
Verified customer
Asked: 2017-05-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PGF2α Receptor (FP) antibody A04716 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-25
Question
Here is the WB image, lot number and protocol we used for liver using anti-PGF2α Receptor (FP) antibody A04716. Please let me know if you require anything else.
J. Mangal
Verified customer
Asked: 2013-03-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-03-04
