Anti-PGAP1 Antibody Picoband™

Product Name

Anti-PGAP1 Antibody Picoband™

View all PGAP1 Antibodies

SKU/Catalog Number

A08640

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PGAP1 Antibody Picoband™ catalog # A08640. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PGAP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08640)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human PGAP1 recombinant protein (Position: K166-R904). Human PGAP1 shares 89% and 88.5% amino acid (aa) sequence identity with mouse and rat PGAP1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08640 is reactive to PGAP1 in Human

Applications

A08640 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

100 kDa, 58 kDa

Calculated molecular weight

52588 MW

Background of PGAP1

Post-GPI attachment to proteins 1 is a protein that in humans is encoded by the PGAP1 gene. The protein encoded by this gene functions early in the glycosylphosphatidylinositol (GPI) biosynthetic pathway, catalyzing the inositol deacylation of GPI. The encoded protein is required for the production of GPI that can attach to proteins, and this may be an important factor in the transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. Defects in this gene are a cause an autosomal recessive form of cognitive impairment.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PGAP1 using anti-PGAP1 antibody (A08640).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGAP1 antigen affinity purified polyclonal antibody (Catalog # A08640) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGAP1 at approximately 100,58 kDa. The expected band size for PGAP1 is at 105,49-53,85-90 kDa.

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Figure 2. Flow Cytometry analysis of 293T cells using anti-PGAP1 antibody (A08640).
Overlay histogram showing 293T cells stained with A08640 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGAP1 Antibody (A08640, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PGAP1 (Source: Uniprot.org, NCBI)

Alternative Names

Bst1; EC 3.1; FLJ12377; FLJ42774; GPI deacylase; GPI inositol-deacylase; hPGAP1; ISPD3024; post-GPI attachment to proteins 1; Post-GPI attachment to proteins factor 1 PGAP1 Bst1, ISPD3024, MRT42, SPG67 post-GPI attachment to proteins inositol deacylase 1 GPI inositol-deacylase|post-GPI attachment to proteins 1|post-GPI attachment to proteins factor 1

For more info on PGAP1, check out the PGAP1 Infographic

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In this infographic, you will see the following information for PGAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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