Anti-PGAP1 Antibody (N-Term)

Product Name

Anti-PGAP1 Antibody (N-Term)

View all PGAP1 Antibodies

SKU/Catalog Number

M08640

Size

50 µl

Description

Boster Bio Anti-PGAP1 Antibody (N-Term) (Catalog # M08640). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-PGAP1 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08640)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This PGAP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 90-122 amino acids from human PGAP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M08640 is reactive to PGAP1 in Human, Mouse, Rat

Applications

M08640 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

105383 Da

Background of PGAP1

Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:2000
FC: 1:25

Validation Images & Assay Conditions

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PGAP1 with M08640 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

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All lanes : Anti-PGAP1 Antibody (N-Term) at 1:2000 dilution
Lane 1: SH-SY5Y whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: 293 whole cell lysate
Lane 4: mouse kidney lysate
Lane 5: mouse testis lysate
Lane 6: rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 105 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Overlay histogram showing Hela cells stained with M08640 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M08640, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight®488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For PGAP1 (Source: Uniprot.org, NCBI)

Alternative Names

Bst1; EC 3.1; FLJ12377; FLJ42774; GPI deacylase; GPI inositol-deacylase; hPGAP1; ISPD3024; post-GPI attachment to proteins 1; Post-GPI attachment to proteins factor 1 PGAP1 Bst1, ISPD3024, MRT42, SPG67 post-GPI attachment to proteins inositol deacylase 1 GPI inositol-deacylase|post-GPI attachment to proteins 1|post-GPI attachment to proteins factor 1

For more info on PGAP1, check out the PGAP1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PGAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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