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Product Info Summary
| SKU: | M02382 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PFKFB3 Monoclonal Antibody
View all 6 Phosphofructo 2 Kinase Antibodies
SKU/Catalog Number
M02382
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PFKFB3 Monoclonal Antibody catalog # M02382. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PFKFB3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02382)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADII-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PFKFB3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02382 is reactive to PFKFB3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
85 kDa
Calculated molecular weight
120839 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02382 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of PFKFB3 expression in Jurkat cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For PFKFB3 (Source: Uniprot.org, NCBI)
Gene Name
PFKFB3
Full Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Weight
120839 MW
Alternative Names
ATP-citrate synthase;2.3.3.8;ATP-citrate (pro-S-)-lyase;ACL;Citrate cleavage enzyme;ACLY; PFKFB3 IPFK2, PFK2, iPFK-2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3|6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase|6PF-2-K/Fru-2,6-P2ase 3|6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme|PFK/FBPase 3|fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase|inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase|renal carcinoma NY-REN-56
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PFKFB3, check out the PFKFB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PFKFB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PFKFB3 Monoclonal Antibody (M02382)
Hello CJ!
M02382 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhao Q,Li J,Wu B,Shang Y,Huang X,Dong H,Liu H,Chen W,Gui R,Nie X.Smart Biomimetic Nanocomposites Mediate Mitochondrial Outcome through Aerobic Glycolysis Reprogramming: A Promising Treatment for Lymphoma.ACS Appl Mater Interfaces.2020 May 20;12(20):22687-
Species: Human,Mouse
M02382 usage in article: APP:IF, SAMPLE:LYMPHOMA TUMOR TISSUE, DILUTION:NA
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Customer Q&As
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2 Customer Q&As for Anti-PFKFB3 Monoclonal Antibody
Question
We are currently using anti-PFKFB3 Monoclonal antibody M02382 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
The anti-PFKFB3 Monoclonal antibody (M02382) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-20
Question
What sample was tested in WB and IHC applications with M02382?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of PFKFB3 expression in Jurkat cell lysate.
Boster Scientific Support
Answered: 2020-01-09
