Click image to see more details
Product Info Summary
| SKU: | PB10011 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PF4 Antibody Picoband®
SKU/Catalog Number
PB10011
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PF4 Antibody Picoband® catalog # PB10011. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10011)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PF4 recombinant protein (Position: E32-S101). Human PF4 shares 75.4% amino acid (aa) sequence identity with both mouse and rat PF4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10011 is reactive to PF4 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
10845 MW
Background of CXCL4/PF4
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10011 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: 293T whole cell, A549 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (PB10011).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates,
Lane 2: A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # PB10011) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 26 kDa. The expected band size for PF4 is at 11 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PF4 (Source: Uniprot.org, NCBI)
Gene Name
PF4
Full Name
Platelet factor 4
Weight
10845 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
Platelet factor 4;PF-4;C-X-C motif chemokine 4;Iroplact;Oncostatin-A;Platelet factor 4, short form;PF4;CXCL4, SCYB4; PF4 CXCL4, PF-4, SCYB4 platelet factor 4 platelet factor 4|C-X-C motif chemokine 4|chemokine (C-X-C motif) ligand 4|iroplact|oncostatin-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PF4, check out the PF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PF4 Antibody Picoband® (PB10011)
Hello CJ!
No publications found for PB10011
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PF4 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PF4 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-PF4 Antibody Picoband®
Question
Is a blocking peptide available for product anti-PF4 antibody (PB10011)?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
We do provide the blocking peptide for product anti-PF4 antibody (PB10011). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-01
Question
My lab would like to test anti-PF4 antibody PB10011 on human mononuclear cell for research purposes, then I may be interested in using anti-PF4 antibody PB10011 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
The products we sell, including anti-PF4 antibody PB10011, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-02
Question
I see that the anti-PF4 antibody PB10011 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-03
Question
Our lab want to know about using your anti-PF4 antibody for leukocyte chemotaxis studies. Has this antibody been tested with western blotting on 293t whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We appreciate your inquiry. This PB10011 anti-PF4 antibody is validated on 293t whole cell lysates, a549 whole cell lysates. It is guaranteed to work for ELISA, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-02
Question
Our team were happy with the WB result of your anti-PF4 antibody. However we have observed positive staining in leukocyte secreted. using this antibody. Is that expected? Could you tell me where is PF4 supposed to be expressed?
R. Williams
Verified customer
Asked: 2019-04-08
Answer
From literature, leukocyte does express PF4. Generally PF4 expresses in secreted. Regarding which tissues have PF4 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 7644496
Boster Scientific Support
Answered: 2019-04-08
Question
We have observed staining in human mononuclear cell. Are there any suggestions? Is anti-PF4 antibody supposed to stain mononuclear cell positively?
Verified Customer
Verified customer
Asked: 2019-02-13
Answer
From what I have seen in literature mononuclear cell does express PF4. From what I have seen in Uniprot.org, PF4 is expressed in mononuclear cell, leukocyte, among other tissues. Regarding which tissues have PF4 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 7644496
Boster Scientific Support
Answered: 2019-02-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mononuclear cell using anti-PF4 antibody PB10011. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-28
Question
See attached the WB image, lot number and protocol we used for mononuclear cell using anti-PF4 antibody PB10011. Please let me know if you require anything else.
Z. Zhang
Verified customer
Asked: 2018-11-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-02
Question
We ordered your anti-PF4 antibody for WB on mononuclear cell a few years ago. I am using human, and I plan to use the antibody for ELISA next. We are interested in examining mononuclear cell as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
C. Gonzalez
Verified customer
Asked: 2018-09-17
Answer
I took a look at the website and datasheets of our anti-PF4 antibody and I see that PB10011 has been validated on human in both WB and ELISA. Thus PB10011 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-17
Question
Do you have a BSA free version of anti-PF4 antibody PB10011 available?
G. Moore
Verified customer
Asked: 2018-05-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PF4 antibody PB10011 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-24
Question
I was wanting to use your anti-PF4 antibody for ELISA for human mononuclear cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human mononuclear cell identification?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
As indicated on the product datasheet, PB10011 anti-PF4 antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human mononuclear cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-22
Question
Does anti-PF4 antibody PB10011 work for ELISA with mononuclear cell?
Verified Customer
Verified customer
Asked: 2018-02-28
Answer
According to the expression profile of mononuclear cell, PF4 is highly expressed in mononuclear cell. So, it is likely that anti-PF4 antibody PB10011 will work for ELISA with mononuclear cell.
Boster Scientific Support
Answered: 2018-02-28
Question
My question regarding product PB10011, anti-PF4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10011 anti-PF4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-31
Question
Is this PB10011 anti-PF4 antibody reactive to the isotypes of PF4?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
The immunogen of PB10011 anti-PF4 antibody is E. coli-derived human PF4 recombinant protein (Position: E32-S101). Human PF4 shares 75.4% amino acid (aa) sequence identity with both mouse and rat PF4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-02
Question
Would PB10011 anti-PF4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Krishna
Verified customer
Asked: 2015-07-31
Answer
As indicated on the product datasheet, PB10011 anti-PF4 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-07-31
Question
We are currently using anti-PF4 antibody PB10011 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
K. Dhar
Verified customer
Asked: 2015-07-29
Answer
The anti-PF4 antibody (PB10011) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-07-29
