Anti-Platelet factor 4 PF4 Antibody Picoband®

Product Name

Anti-Platelet factor 4 PF4 Antibody Picoband®

View all CXCL4/PF4 Antibodies

SKU/Catalog Number

PA1446

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Platelet factor 4 PF4 Antibody catalog # PA1446. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Platelet factor 4 PF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1446)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PF4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1446 is reactive to PF4 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

50 kDa

Calculated molecular weight

10845 MW

Background of CXCL4/PF4

CXCL4, Chemokine (C-X-C motif) ligand 4, is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. CXCL4 belongs to the CXC chemokine family. The CXCL4 gene contains 3 exons spanning approximately 1 kb. The gene is encoded on a 10-kb EcoRI fragment of genomic DNA. By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1446 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Positive Control

IHC: Human Lung Cancer Tissue

Validation Images & Assay Conditions

Gene/Protein Information For PF4 (Source: Uniprot.org, NCBI)

Alternative Names

Platelet factor 4;PF-4;C-X-C motif chemokine 4;Iroplact;Oncostatin-A;Platelet factor 4, short form;PF4;CXCL4, SCYB4; PF4 CXCL4, PF-4, SCYB4 platelet factor 4 platelet factor 4|C-X-C motif chemokine 4|chemokine (C-X-C motif) ligand 4|iroplact|oncostatin-A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis