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Product Info Summary
| SKU: | PB9384 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Peroxiredoxin 5/PRDX5 Antibody Picoband®
View all Peroxiredoxin 5 Antibodies
SKU/Catalog Number
PB9384
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Peroxiredoxin 5/PRDX5 Antibody Picoband® catalog # PB9384. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Peroxiredoxin 5/PRDX5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9384)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Peroxiredoxin 5 recombinant protein (Position: E66-D198). Human Peroxiredoxin 5 shares 91% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9384 is reactive to PRDX5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
22086 MW
Background of Peroxiredoxin 5
PRDX5 (peroxiredoxin 5) also known as AOEB166, ACR1,B166, MGC117264, MGC142283, MGC142285, PLP, PMP20, PRDX6, PRXV, SBBI10, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. The PRDX5 gene is mapped to11q13.1. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that PRDX5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. PRDX5 mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. PRDX5 plays a protective role against oxidative stress in human cartilage.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9384 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: A549 Whole Cell, Rat Brain Tissue, Mouse Brain Tissue
IHC: Human Lung Cancer Tissue, Rat Intestine Tissue, Mouse Intestine Tissue
ICC/IF: HELA cell
ICC: SMMC cell
FCM: A549 cell
Validation Images & Assay Conditions
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Anti-Peroxiredoxin 5 Picoband antibody, PB9384, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti-Peroxiredoxin 5 Picoband antibody, PB9384, IHC(P)
IHC(P): Rat Intestine Tissue
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Anti-Peroxiredoxin 5 Picoband antibody, PB9384, IHC(P)
IHC(P): Mouse Intestine Tissue
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Anti-Peroxiredoxin 5 Picoband antibody, PB9384, Western blotting
All lanes: Anti Peroxiredoxin 5 (PB9384) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
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Figure 5. IHC analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody (PB9384).
Peroxiredoxin 5 was detected in immunocytochemical section of SMMC Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Peroxiredoxin 5 Antibody (PB9384) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 6. IF analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody (PB9384).
Peroxiredoxin 5 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Peroxiredoxin 5 Antibody (PB9384) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 7. Flow Cytometry analysis of A549 cells using anti-Peroxiredoxin 5 antibody (PB9384).
Overlay histogram showing A549 cells stained with PB9384 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 5 Antibody (PB9384,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PRDX5 (Source: Uniprot.org, NCBI)
Gene Name
PRDX5
Full Name
Peroxiredoxin-5, mitochondrial
Weight
22086 MW
Superfamily
peroxiredoxin family
Alternative Names
Peroxiredoxin-5, mitochondrial;1.11.1.15;Alu corepressor 1;Antioxidant enzyme B166;AOEB166;Liver tissue 2D-page spot 71B;PLP;Peroxiredoxin V;Prx-V;Peroxisomal antioxidant enzyme;TPx type VI;Thioredoxin peroxidase PMP20;Thioredoxin reductase;PRDX5;ACR1;SBBI10; PRDX5 ACR1, AOEB166, B166, HEL-S-55, PLP, PMP20, PRDX6, PRXV, SBBI10, prx-V peroxiredoxin 5 peroxiredoxin-5, mitochondrial|Alu co-repressor 1|TPx type VI|antioxidant enzyme B166|epididymis secretory protein Li 55|liver tissue 2D-page spot 71B|peroxiredoxin V|peroxisomal antioxidant enzyme|thioredoxin peroxidase PMP20|thioredoxin reductase|thioredoxin-dependent peroxiredoxin 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRDX5, check out the PRDX5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRDX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Peroxiredoxin 5/PRDX5 Antibody Picoband® (PB9384)
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Customer Q&As
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1 Customer Q&As for Anti-Peroxiredoxin 5/PRDX5 Antibody Picoband®
Question
We are currently using anti-Peroxiredoxin 5/PRDX5 antibody PB9384 for rat tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The anti-Peroxiredoxin 5/PRDX5 antibody (PB9384) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-18
