Click image to see more details
Product Info Summary
| SKU: | A03672-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PEPT1 SLC15A1 Antibody
View all PEPT1/SLC15A1 Antibodies
SKU/Catalog Number
A03672-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PEPT1 SLC15A1 Antibody catalog # A03672-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PEPT1 SLC15A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03672-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SLC15A1 at amino acid range 225-274
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03672-1 is reactive to SLC15A1 in Human, Mouse, Rat
Applications
A03672-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
78806 MW
Background of PEPT1/SLC15A1
CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of Mouse Brain Mouse Heart Mouse Lung Mouse Kidney cells using PEPT1 Polyclonal antibody.
Click image to see more details
Western blot analysis of SLC15A1 Antibody. The lane on the right is blocked with the SLC15A1 peptide.
Click image to see more details
Western blot analysis of the lysates from HeLa cells using SLC15A1 antibody.
Protein Target Info & Infographic
Gene/Protein Information For SLC15A1 (Source: Uniprot.org, NCBI)
Gene Name
SLC15A1
Full Name
Solute carrier family 15 member 1
Weight
78806 MW
Superfamily
PTR2/POT transporter (TC 2.A.17) family
Alternative Names
Caco-2 oligopeptide transporter; HPECT1macrophage oligopeptide transporter PEPT1; HPEPT1; Intestinal H(+)/peptide cotransporter; Oligopeptide transporter, small intestine isoform; PEPT1; PEPT1intestinal H+/peptide cotransporter; Peptide transporter 1; SLC15A1; solute carrier family 15 (oligopeptide transporter), member 1; solute carrier family 15 member 1 SLC15A1 HPECT1, HPEPT1, PEPT1 solute carrier family 15 member 1 solute carrier family 15 member 1|Caco-2 oligopeptide transporter|intestinal H+/peptide cotransporter|macrophage oligopeptide transporter PEPT1|peptide transporter 1|solute carrier family 15 (oligopeptide transporter), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC15A1, check out the SLC15A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC15A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PEPT1 SLC15A1 Antibody (A03672-1)
Hello CJ!
No publications found for A03672-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PEPT1 SLC15A1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PEPT1 SLC15A1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-PEPT1 SLC15A1 Antibody
Question
Does A03672-1 anti-PEPT1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
As indicated on the product datasheet, A03672-1 anti-PEPT1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-14
Question
Would anti-PEPT1 antibody A03672-1 work on canine WB with duodenum?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Our lab technicians have not tested anti-PEPT1 antibody A03672-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PEPT1 antibody A03672-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine duodenum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-14
Question
We are currently using anti-PEPT1 antibody A03672-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
The anti-PEPT1 antibody (A03672-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-22
Question
My lab would like to test anti-PEPT1 antibody A03672-1 on rat intestine for research purposes, then I may be interested in using anti-PEPT1 antibody A03672-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Thomas
Verified customer
Asked: 2014-11-26
Answer
The products we sell, including anti-PEPT1 antibody A03672-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-11-26
