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Product Info Summary
| SKU: | A05921 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Pellino 1 PELI1 Antibody
SKU/Catalog Number
A05921
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Pellino 1 PELI1 Antibody (Catalog # A05921). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Pellino 1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Pellino 1 PELI1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05921)
Host
Rabbit
Contents
Pellino 1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal Pellino 1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Pellino 1. The immunogen is located within amino acids 300 - 350 of Pellino 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SEC62 antibody is predicted to not cross-react with other SEC proteins. Multiple isoforms of SEC62 are known to exist.
Reactive Species
A05921 is reactive to PELI1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
46286 MW
Background of Pellino 1
The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-κB. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-ε and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05921 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Pellino 1 antibody can be used for detection of Pellino 1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Pellino 1 in human liver tissue lysate with Pellino 1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of Pellino in HepG2 cells with Pellino 1 antibody at 2.5 μg/ml.
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Immunofluorescence of Pellino 1 in HepG2 cells with Pellino 1 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For PELI1 (Source: Uniprot.org, NCBI)
Gene Name
PELI1
Full Name
E3 ubiquitin-protein ligase pellino homolog 1
Weight
46286 MW
Superfamily
pellino family
Alternative Names
PRISM, E3 ubiquitin-protein ligase pellino homolog 1, Pellino-related intracellular-signaling molecule, Pellino-1 PELI1 pellino E3 ubiquitin protein ligase 1 E3 ubiquitin-protein ligase pellino homolog 1|RING-type E3 ubiquitin transferase pellino homolog 1|pellino homolog 1|pellino-1|pellino-related intracellular-signaling molecule|protein pellino homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PELI1, check out the PELI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PELI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Pellino 1 PELI1 Antibody (A05921)
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