Anti-Pea3/ETV4 Antibody Picoband®

Product Name

Anti-Pea3/ETV4 Antibody Picoband®

View all Pea3 Antibodies

SKU/Catalog Number

PB9699

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Pea3/ETV4 Antibody Picoband® catalog # PB9699. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Pea3/ETV4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9699)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Pea3, different from the related mouse sequence by seven amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9699 is reactive to ETV4 in Human, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

68 kDa

Calculated molecular weight

53938 MW

Background of Pea3

ETS translocation variant 4  (ETV4), also known as polyoma enhancer activator 3  (PEA3), or E1AF, is a member of the PEA3 subfamily of Ets transcription factors. It is mapped to 17q21.31. E1AF can activate the promoters of various matrix metalloproteinases, genes whose expression is associated with tumor cell invasion and metastasis, by 10 to 20 fold. Pea3 is detected in cells of epithelial and fibroblastic origin. It is also a transcriptional activator that binds to the enhancer of the adenovirus E1A gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9699 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Positive Control

WB: Rat Lung Tissue, HELA Whole Cell, A549 Whole Cell, SW620 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For ETV4 (Source: Uniprot.org, NCBI)

Alternative Names

ETS translocation variant 4;Adenovirus E1A enhancer-binding protein;E1A-F;Polyomavirus enhancer activator 3 homolog;Protein PEA3;ETV4;E1AF, PEA3; ETV4 E1A-F, E1AF, PEA3, PEAS3 ETS variant transcription factor 4 ETS translocation variant 4|ETS variant 4|EWS protein/E1A enhancer binding protein chimera|adenovirus E1A enhancer-binding protein|ets variant gene 4 (E1A enhancer-binding protein, E1AF)|polyomavirus enhancer activator 3 homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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