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Product Info Summary
| SKU: | A00408-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-GATA1 (Ab-142) Antibody
SKU/Catalog Number
A00408-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GATA1 (Ab-142) Antibody (Catalog # A00408-1). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GATA1 (Ab-142) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00408-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.140~144 (R-L-S-P-D) derived from Human GATA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00408-1 is reactive to GATA1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
79663 MW
Background of GATA-1
GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.
Zon LI, et al. (1990) Proc Natl Acad. Sci USA. 87: 668-672.
Trainor C D, et al. (1990) Nature. 343: 92-96.
Nichols K E, et al. (2000) Nat Genet. 24: 266-270.
Freson K, et al. (2001) Blood. 98: 85-92.
Mehaffey M G, et al. (2001) Blood. 98: 2681-2688.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00408-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GATA1 using anti-GATA1 antibody (A00408-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA1 antigen affinity purified polyclonal antibody (Catalog # A00408-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for GATA1.
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Immunofluorescence staining of methanol-fixed Hela cells using GATA1(Ab-142) Antibody #A00408-1.
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Figure 2. IHC analysis of GATA1 using anti-GATA1 antibody (A00408-1).
GATA1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GATA1 Antibody (A00408-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GATA1 (Source: Uniprot.org, NCBI)
Gene Name
GATA1
Full Name
Erythroid transcription factor
Weight
79663 MW
Alternative Names
F-box/WD repeat-containing protein 7 ;Archipelago homolog GATA1 ERYF1, GATA-1, GF-1, GF1, NF-E1, NFE1, XLANP, XLTDA, XLTT GATA binding protein 1 erythroid transcription factor|GATA-binding factor 1|NF-E1 DNA-binding protein|erythroid transcription factor 1|globin transcription factor 1|nuclear factor, erythroid 1|transcription factor GATA1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GATA1, check out the GATA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GATA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GATA1 (Ab-142) Antibody (A00408-1)
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