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Product Info Summary
| SKU: | M06683-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PDXK Mouse Monoclonal Antibody [Clone ID: OTI5H5]
View all Pyridoxal Kinase/PDXK Antibodies
SKU/Catalog Number
M06683-1
Size
100 µl
Description
Boster Bio PDXK mouse monoclonal antibody, clone OTI5H5 (formerly 5H5). Catalog# M06683-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PDXK Mouse Monoclonal Antibody [Clone ID: OTI5H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06683-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5H5
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human PDXK (NP_03672) produced in HEK293 cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06683-1 is reactive to PDXK in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
35.102kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06683-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDXK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDXK.
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Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-PDXK monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either PDXK (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDXK antibody (M06683-1)
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Anti-PDXK mouse monoclonal antibody (M06683-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDXK.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT) and PDXK-Knockout HEK293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PDXK monoclonal antibody M06683-1 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Protein Target Info & Infographic
Gene/Protein Information For PDXK (Source: Uniprot.org, NCBI)
Gene Name
PDXK
Full Name
Pyridoxal kinase
Weight
35.102kDa
Superfamily
pyridoxine kinase family
Alternative Names
C21orf124; C21orf97; chromosome 21 open reading frame 124; DKFZp566A071; EC 2.7.1.35; FLJ21324; FLJ37311; HEL-S-1a; MGC15873; MGC31754; MGC52346; PDXK; PKH; PKHchromosome 21 open reading frame 97; PNK; PNKhuman pyridoxal kinase, EC 2.7.1.3510FLJ31940; PRED79; pyridoxal (pyridoxine, vitamin B6) kinase; Pyridoxal Kinase; pyridoxamine kinase; Pyridoxine kinase; Vitamin B6 Kinase PDXK C21orf124, C21orf97, HEL-S-1a, HMSN6C, PKH, PNK, PRED79 pyridoxal kinase pyridoxal kinase|epididymis secretory sperm binding protein Li 1a|pyridoxal (pyridoxine, vitamin B6) kinase|pyridoxamine kinase|pyridoxine kinase|vitamin B6 kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDXK, check out the PDXK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDXK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDXK Mouse Monoclonal Antibody [Clone ID: OTI5H5] (M06683-1)
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