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Product Info Summary
| SKU: | M06683 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-PDXK Antibody (N-term)
View all Pyridoxal Kinase/PDXK Antibodies
SKU/Catalog Number
M06683
Size
50 µl
Description
Boster Bio Anti-PDXK Antibody (N-term) (Catalog # M06683). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PDXK Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06683)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1007CT2.1.1
Isotype
IgG1,k
Immunogen
This PDXK antibody is generated from a mouse immunized with a recombinant protein between 1-312 amino acids from human PDXK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06683 is reactive to PDXK in Human
Reconstitution
Calculated molecular weight
35102 Da
Background of Pyridoxal Kinase/PDXK
Required for synthesis of pyridoxal-5-phosphate from vitamin B6.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06683 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-PDXK Antibody (N-term) at 1:4000 dilution
Lane 1: 293 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: U-251 MG whole cell lysate
Lane 4: Human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 35 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M06683 staining PDXK in human testis tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For PDXK (Source: Uniprot.org, NCBI)
Gene Name
PDXK
Full Name
Pyridoxal kinase
Weight
35102 Da
Superfamily
pyridoxine kinase family
Alternative Names
Pyridoxal kinase, 2.7.1.35, Pyridoxine kinase, PDXK, C21orf124, C21orf97, PKH, PNK PDXK C21orf124, C21orf97, HEL-S-1a, HMSN6C, PKH, PNK, PRED79 pyridoxal kinase pyridoxal kinase|epididymis secretory sperm binding protein Li 1a|pyridoxal (pyridoxine, vitamin B6) kinase|pyridoxamine kinase|pyridoxine kinase|vitamin B6 kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDXK, check out the PDXK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDXK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDXK Antibody (N-term) (M06683)
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