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Product Info Summary
| SKU: | M01159 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PDPK1/Pdk 1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01159
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PDPK1/Pdk 1 Rabbit Monoclonal Antibody catalog # M01159. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDPK1/Pdk 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01159)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HOO-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PDPK1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01159 is reactive to PDPK1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
63152 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01159 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
Positive Control
WB: HEK293 cell
IHC: human kidney tissue
ICC/IF: PC-12 cell
Validation Images & Assay Conditions
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Western blot analysis of PDPK1 expression in HEK293 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human kidney, using PDPK1 Antibody.
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Immunofluorescent analysis of PC-12 cells, using PDPK1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For PDPK1 (Source: Uniprot.org, NCBI)
Gene Name
PDPK1
Full Name
3-phosphoinositide-dependent protein kinase 1
Weight
63152 MW
Superfamily
protein kinase superfamily
Alternative Names
3-phosphoinositide-dependent protein kinase 1;hPDK1;2.7.11.1;PDPK1;PDK1; PDPK1 PDK1, PDPK2, PDPK2P, PRO0461 3-phosphoinositide dependent protein kinase 1 3-phosphoinositide-dependent protein kinase 1|3-phosphoinositide-dependent protein kinase 2 pseudogene|PkB kinase like gene 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDPK1, check out the PDPK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDPK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDPK1/Pdk 1 Rabbit Monoclonal Antibody (M01159)
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No publications found for M01159
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-PDPK1/Pdk 1 Rabbit Monoclonal Antibody
Question
Is there a BSA free version of anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody M01159 available?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody M01159 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-11
Question
I see that the anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody M01159 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-27
Question
Does anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody M01159 work on monkey IF with embryonic kidney?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
Our lab technicians have not tested anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody M01159 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody M01159 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey embryonic kidney in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-29
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney uterus using anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody M01159. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-11
Question
Is a blocking peptide available for product anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody (M01159)?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
We do provide the blocking peptide for product anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody (M01159). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-03
Question
My lab would like to test anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody M01159 on mouse kidney uterus for research purposes, then I may be interested in using anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody M01159 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-18
Answer
The products we sell, including anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody M01159, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-18
Question
I was wanting to use your anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody for WB for mouse kidney uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse kidney uterus identification?
A. Moore
Verified customer
Asked: 2014-04-15
Answer
It shows on the product datasheet, M01159 anti-PDPK1/Pdk 1 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse kidney uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-04-15
