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Product Info Summary
| SKU: | M03813 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PDIA6/Erp5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03813
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PDIA6/Erp5 Rabbit Monoclonal Antibody catalog # M03813. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDIA6/Erp5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03813)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOBE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PDIA6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03813 is reactive to PDIA6 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
43 kDa
Calculated molecular weight
48121 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03813 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of PDIA6 expression in K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PDIA6 (Source: Uniprot.org, NCBI)
Gene Name
PDIA6
Full Name
Protein disulfide-isomerase A6
Weight
48121 MW
Superfamily
protein disulfide isomerase family
Alternative Names
Protein disulfide-isomerase A6;5.3.4.1;Endoplasmic reticulum protein 5;ER protein 5;ERp5;Protein disulfide isomerase P5;Thioredoxin domain-containing protein 7;PDIA6;ERP5, P5, TXNDC7; PDIA6 ERP5, P5, TXNDC7 protein disulfide isomerase family A member 6 protein disulfide-isomerase A6|ER protein 5|endoplasmic reticulum protein 5|epididymis secretory sperm binding protein|protein disulfide isomerase P5|protein disulfide isomerase-associated 6|protein disulfide isomerase-related protein|thioredoxin domain containing 7 (protein disulfide isomerase)|thioredoxin domain-containing protein 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDIA6, check out the PDIA6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDIA6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDIA6/Erp5 Rabbit Monoclonal Antibody (M03813)
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No publications found for M03813
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Customer Q&As
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6 Customer Q&As for Anti-PDIA6/Erp5 Rabbit Monoclonal Antibody
Question
Can you help my question with product M03813, anti-PDIA6/Erp5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03813 anti-PDIA6/Erp5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-30
Question
Here is the WB image, lot number and protocol we used for melanoma using anti-PDIA6/Erp5 Rabbit Monoclonal antibody M03813. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-02
Question
We are currently using anti-PDIA6/Erp5 Rabbit Monoclonal antibody M03813 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The anti-PDIA6/Erp5 Rabbit Monoclonal antibody (M03813) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-04
Question
I see that the anti-PDIA6/Erp5 Rabbit Monoclonal antibody M03813 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-21
Question
Is there a BSA free version of anti-PDIA6/Erp5 Rabbit Monoclonal antibody M03813 available?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PDIA6/Erp5 Rabbit Monoclonal antibody M03813 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-17
Question
Is this M03813 anti-PDIA6/Erp5 Rabbit Monoclonal antibody reactive to the isotypes of PDIA6?
G. Parker
Verified customer
Asked: 2016-04-19
Answer
The immunogen of M03813 anti-PDIA6/Erp5 Rabbit Monoclonal antibody is A synthesized peptide derived from human PDIA6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-04-19
