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Product Info Summary
| SKU: | E02335 |
|---|---|
| Size: | 200μg/vial |
| Reactive Species: | Bovine, Chicken, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus, Porcine |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-PDI P4hb Antibody (Monoclonal, 1D3), PE Conjugated
View all Protein Disulfide Isomerase/P4HB Antibodies
SKU/Catalog Number
E02335
Size
200μg/vial
Form
Liquid
Description
Boster Bio Anti-PDI P4hb Antibody (Monoclonal, 1D3), PE Conjugated (Catalog# E02335). Tested in Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDI P4hb Antibody (Monoclonal, 1D3), PE Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # E02335)
Host
Mouse
Contents
Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
Clonality
Monoclonal
Clone Number
1D3
Isotype
IgG1
Immunogen
Synthetic peptide corresponding to the sequence near the C-terminus of rat PDI
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
E02335 is reactive to P4hb in Bovine, Chicken, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus, Porcine
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
56951 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
E02335 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1:100)
Detects a band of ~58kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Flow cytometry analysis of 106 Jurkat cells stained using PDI mAb (1D3), R-Phycoerythrin Conjugate at a concentration of 10μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For P4hb (Source: Uniprot.org, NCBI)
Gene Name
P4hb
Full Name
Protein disulfide-isomerase
Weight
56951 MW
Superfamily
protein disulfide isomerase family
Alternative Names
Protein disulfide-isomerase;PDI;5.3.4.1;Cellular thyroid hormone-binding protein;Prolyl 4-hydroxylase subunit beta;P4hb;Pdia1; P4HB CLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB prolyl 4-hydroxylase subunit beta protein disulfide-isomerase|cellular thyroid hormone-binding protein|collagen prolyl 4-hydroxylase beta|glutathione-insulin transhydrogenase|p55|procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide|prolyl 4-hydroxylase, beta polypeptide|protein disulfide isomerase family A, member 1|protein disulfide isomerase-associated 1|protein disulfide isomerase/oxidoreductase|protocollagen hydroxylase|testicular secretory protein Li 32|thyroid hormone-binding protein p55
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on P4hb, check out the P4hb Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for P4hb: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDI P4hb Antibody (Monoclonal, 1D3), PE Conjugated (E02335)
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No publications found for E02335
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-PDI P4hb Antibody (Monoclonal, 1D3), PE Conjugated
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung skin using anti-PDI antibody (Monoclonal, 1D3), PE Conjugated E02335. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-30
Question
We are currently using anti-PDI antibody (Monoclonal, 1D3), PE Conjugated E02335 for mouse tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
The anti-PDI antibody (Monoclonal, 1D3), PE Conjugated (E02335) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-26
Question
My question regarding product E02335, anti-PDI antibody (Monoclonal, 1D3), PE Conjugated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free E02335 anti-PDI antibody (Monoclonal, 1D3), PE Conjugated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-06
