Anti-PDGF Receptor Beta PDGFRB Antibody (Monoclonal, 42G12)

Product Name

Anti-PDGF Receptor Beta PDGFRB Antibody (Monoclonal, 42G12)

View all PDGF R beta Antibodies

SKU/Catalog Number

M00096

Size

100μg/vial

Form

Liquid

Description

Boster Bio Anti-PDGF Receptor Beta PDGFRB Antibody (Monoclonal, 42G12) (Catalog# M00096). Tested in IHC-P, WB application(s). This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Cite This Product

Anti-PDGF Receptor Beta PDGFRB Antibody (Monoclonal, 42G12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00096)

Host

Mouse

Contents

Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Clonality

Monoclonal

Clone Number

42G12

Isotype

IgG1

Immunogen

Recombinant human PDGF Receptor β

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Weakly cross-reacts with human PDGF receptor β.

Reactive Species

M00096 is reactive to PDGFRB in Mouse, Rat

Applications

M00096 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

123968 MW

Background of PDGF R beta

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1µg/ml, ECL). Detects a band of ~125kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of PDGF Receptor Beta expression in MW marker extract (lane 1) and mouse cardiac muscle extract (lane 2). PDGF Receptor Beta at 125KD was detected using mouse anti-PDGF Receptor Beta Antigen Affinity purified monoclonal antibody (Catalog # M00096) at 1 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).

Click image to see more details

PDGF Receptor Beta was detected in paraffin-embedded sections of human placenta tissues using mouse anti-PDGF Receptor Beta Antigen Affinity purified monoclonal antibody (Catalog # M00096) at 20 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1021).

Click image to see more details

PDGF Receptor Beta was detected in paraffin-embedded sections of human spleen tissues using mouse anti-PDGF Receptor Beta Antigen Affinity purified monoclonal antibody (Catalog # M00096) at 20 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1021).

Gene/Protein Information For PDGFRB (Source: Uniprot.org, NCBI)

Alternative Names

beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide PDGFRB CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT platelet derived growth factor receptor beta platelet-derived growth factor receptor beta|Activated tyrosine kinase PDGFRB|CD140 antigen-like family member B|NDEL1-PDGFRB|PDGF-R-beta|PDGFR-beta|beta-type platelet-derived growth factor receptor|platelet-derived growth factor receptor 1|platelet-derived growth factor receptor, beta polypeptide

For more info on PDGFRB, check out the PDGFRB Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PDGFRB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the PDGFRB Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis