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Product Info Summary
| SKU: | M00096 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-PDGF Receptor Beta PDGFRB Antibody (Monoclonal, 42G12)
View all PDGF R beta Antibodies
SKU/Catalog Number
M00096
Size
100μg/vial
Form
Liquid
Description
Boster Bio Anti-PDGF Receptor Beta PDGFRB Antibody (Monoclonal, 42G12) (Catalog# M00096). Tested in IHC-P, WB application(s). This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Cite This Product
Anti-PDGF Receptor Beta PDGFRB Antibody (Monoclonal, 42G12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00096)
Host
Mouse
Contents
Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
42G12
Isotype
IgG1
Immunogen
Recombinant human PDGF Receptor β
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Weakly cross-reacts with human PDGF receptor β.
Reactive Species
M00096 is reactive to PDGFRB in Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
123968 MW
Background of PDGF R beta
Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00096 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1µg/ml, ECL). Detects a band of ~125kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Western blot analysis of PDGF Receptor Beta expression in MW marker extract (lane 1) and mouse cardiac muscle extract (lane 2). PDGF Receptor Beta at 125KD was detected using mouse anti-PDGF Receptor Beta Antigen Affinity purified monoclonal antibody (Catalog # M00096) at 1 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
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PDGF Receptor Beta was detected in paraffin-embedded sections of human placenta tissues using mouse anti-PDGF Receptor Beta Antigen Affinity purified monoclonal antibody (Catalog # M00096) at 20 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1021).
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PDGF Receptor Beta was detected in paraffin-embedded sections of human spleen tissues using mouse anti-PDGF Receptor Beta Antigen Affinity purified monoclonal antibody (Catalog # M00096) at 20 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1021).
Protein Target Info & Infographic
Gene/Protein Information For PDGFRB (Source: Uniprot.org, NCBI)
Gene Name
PDGFRB
Full Name
Platelet-derived growth factor receptor beta
Weight
123968 MW
Superfamily
protein kinase superfamily
Alternative Names
Platelet-derived growth factor receptor beta;PDGF-R-beta;PDGFR-beta;2.7.10.1;Beta platelet-derived growth factor receptor;Beta-type platelet-derived growth factor receptor;CD140 antigen-like family member B;Platelet-derived growth factor receptor 1;PDGFR-1;CD140b;PDGFRB;PDGFR, PDGFR1; PDGFRB CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT platelet derived growth factor receptor beta platelet-derived growth factor receptor beta|Activated tyrosine kinase PDGFRB|CD140 -like family member B|NDEL1-PDGFRB|PDGF-R-beta|PDGFR-beta|beta-type platelet-derived growth factor receptor|platelet-derived growth factor receptor 1|platelet-derived growth factor receptor, beta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDGFRB, check out the PDGFRB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDGFRB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDGF Receptor Beta PDGFRB Antibody (Monoclonal, 42G12) (M00096)
Hello CJ!
M00096 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Changes in the expression of platelet-derived growth factor in astrocytes in diabetic rats with spinal cord injury
RNA interference targeting the platelet-derived growth factor receptor ? subunit ameliorates experimental hepatic fibrosis in rats
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-PDGF Receptor Beta PDGFRB Antibody (Monoclonal, 42G12)
Question
I was wanting to use your anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) for IHC for mouse ectocervix on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse ectocervix identification?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
It shows on the product datasheet, M00096 anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) has been validated for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse ectocervix in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-29
Question
We ordered your anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) for WB on ectocervix in the past. I am using rat, and We intend to use the antibody for IHC next. I am looking for examining ectocervix as well as brain in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
I have checked the website and datasheets of our anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) and it appears that M00096 has been tested on rat in both WB and IHC. Thus M00096 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-14
Question
Is this M00096 anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) reactive to the isotypes of PDGFRB?
D. Collins
Verified customer
Asked: 2019-04-26
Answer
The immunogen of M00096 anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) is Recombinant human PDGF Receptor beta . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-26
Question
My team were content with the WB result of your anti-PDGF Receptor Beta antibody (Monoclonal, 42G12). However we have observed positive staining in ectocervix cell membrane using this antibody. Is that expected? Could you tell me where is PDGFRB supposed to be expressed?
T. Mangal
Verified customer
Asked: 2018-09-03
Answer
Based on literature, ectocervix does express PDGFRB. Generally PDGFRB expresses in cell membrane. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-09-03
Question
We have seen staining in rat ectocervix. Any tips? Is anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) supposed to stain ectocervix positively?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
From literature ectocervix does express PDGFRB. From Uniprot.org, PDGFRB is expressed in ectocervix, brain, among other tissues. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-08-13
Question
We are currently using anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) M00096 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
The anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) (M00096) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-01
Question
We need to test anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) M00096 on mouse ectocervix for research purposes, then I may be interested in using anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) M00096 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Rodriguez
Verified customer
Asked: 2014-12-25
Answer
The products we sell, including anti-PDGF Receptor Beta antibody (Monoclonal, 42G12) M00096, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-12-25
