Product Info Summary
SKU: | A05304-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PDE8A Antibody Picoband™
SKU/Catalog Number
A05304-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PDE8A Antibody Picoband™ catalog # A05304-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PDE8A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05304-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human PDE8A recombinant protein (Position: R32-E495). Human PDE8A shares 80.5% amino acid (aa) sequence identity with mouse PDE8A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05304-1 is reactive to PDE8A in Human
Applications
A05304-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
93 kDa
Calculated molecular weight
52588 MW
Background of PDE8A
High affinity cAMP-specific and IBMX-insensitive 3', 5'-cyclic phosphodiesterase 8A is an enzyme that in humans is encoded by the PDE8A gene. The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PDE8A using anti-PDE8A antibody (A05304-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE8A antigen affinity purified polyclonal antibody (Catalog # A05304-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE8A at approximately 93 kDa. The expected band size for PDE8A is at 93 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-PDE8A antibody (A05304-1).
Overlay histogram showing SH-SY5Y cells stained with A05304-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE8A Antibody (A05304-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PDE8A (Source: Uniprot.org, NCBI)
Gene Name
PDE8A
Full Name
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Weight
52588 MW
Superfamily
cyclic nucleotide phosphodiesterase family
Alternative Names
cAMP-specific cyclic nucleotide phosphodiesterase 8A; EC 3.1.4.17; FLJ16150; high affinity cAMP-specific and IBMX-insensitive 3'-5'-cyclic phosphodiesterase8A; high-affinity cAMP-specific and IBMX-insensitive 3'-5'-cyclic phosphodiesterase8A; HsT19550; phosphodiesterase 8A PDE8A HsT19550 phosphodiesterase 8A high affinity cAMP-specific and IBMX-insensitive 3,5-cyclic phosphodiesterase 8A|cAMP-specific cyclic nucleotide phosphodiesterase 8A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDE8A, check out the PDE8A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDE8A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDE8A Antibody Picoband™ (A05304-1)
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