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Product Info Summary
| SKU: | M01105 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PDCD4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01105
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PDCD4 Rabbit Monoclonal Antibody catalog # M01105. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDCD4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01105)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACAE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PDCD4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01105 is reactive to PDCD4 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
80-100 kDa
Calculated molecular weight
84660 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01105 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of PDCD4 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PDCD4 (Source: Uniprot.org, NCBI)
Gene Name
PDCD4
Full Name
Programmed cell death protein 4
Weight
84660 MW
Superfamily
PDCD4 family
Alternative Names
Heat shock protein HSP 90-alpha;Heat shock 86 kDa;HSP 86;HSP86;Lipopolysaccharide-associated protein 2;LAP-2;LPS-associated protein 2;Renal carcinoma antigen NY-REN-38;HSP90AA1;HSP90A, HSPC1, HSPCA; Pdcd4|D19Ucl, D19Ucla1, MA-, Ma3, T, Tis|programmed cell death 4|programmed cell death protein 4|protein MA-3|topoisomerase-inhibitor suppressed protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDCD4, check out the PDCD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDCD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDCD4 Rabbit Monoclonal Antibody (M01105)
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No publications found for M01105
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Customer Q&As
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5 Customer Q&As for Anti-PDCD4 Rabbit Monoclonal Antibody
Question
Will M01105 anti-PDCD4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Williams
Verified customer
Asked: 2020-03-27
Answer
You can see on the product datasheet, M01105 anti-PDCD4 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-27
Question
What sample was used in your WB analysis of M01105?
Verified customer
Asked: 2019-12-18
Answer
For the Anti-PDCD4 Rabbit Monoclonal Antibody (M01105), western blot analysis of PDCD4 expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2019-12-20
Question
I was wanting to use your anti-PDCD4 Monoclonal antibody for IF for mouse body of pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse body of pancreas identification?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
As indicated on the product datasheet, M01105 anti-PDCD4 Monoclonal antibody has been validated for IF on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse body of pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-14
Question
Here is the WB image, lot number and protocol we used for body of pancreas using anti-PDCD4 Monoclonal antibody M01105. Please let me know if you require anything else.
D. Wu
Verified customer
Asked: 2019-02-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-15
Question
Does anti-PDCD4 Monoclonal antibody M01105 work for IF with body of pancreas?
A. Brown
Verified customer
Asked: 2019-01-28
Answer
According to the expression profile of body of pancreas, PDCD4 is highly expressed in body of pancreas. So, it is likely that anti-PDCD4 Monoclonal antibody M01105 will work for IF with body of pancreas.
Boster Scientific Support
Answered: 2019-01-28
