Product Info Summary
SKU: | A01105 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-PDCD4 Antibody Picoband®
SKU/Catalog Number
A01105
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PDCD4 Antibody Picoband® catalog # A01105. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDCD4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01105)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PDCD4 recombinant protein (Position: E346-L446). Human PDCD4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat PDCD4, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01105 is reactive to PDCD4 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
51735 MW
Background of Pdcd4
Programmed cell death protein 4 (PDCD4) is a protein that in humans is encoded by the PDCD4 gene. It is mapped to 10q25.2. This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. It also encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01105 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Daudi whole cell, human Hela whole cell, human 293T whole cell
IHC: human appendiceal adenocarcinoma tissue, human gall bladder adenosquamous carcinomaa tissue, human gastric adenocarcinoma tissue
FCM: HepG2 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Click image to see more details
Figure 2. IHC analysis of PDCD4 using anti-PDCD4 antibody (A01105).
PDCD4 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDCD4 Antibody (A01105) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of PDCD4 using anti-PDCD4 antibody (A01105).
PDCD4 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinomaa tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDCD4 Antibody (A01105) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of PDCD4 using anti-PDCD4 antibody (A01105).
PDCD4 was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDCD4 Antibody (A01105) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of HepG2 cells using anti-PDCD4 antibody (A01105).
Overlay histogram showing HepG2 cells stained with A01105 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDCD4 Antibody (A01105, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PDCD4 (Source: Uniprot.org, NCBI)
Gene Name
PDCD4
Full Name
Programmed cell death protein 4
Weight
51735 MW
Superfamily
PDCD4 family
Alternative Names
Programmed cell death protein 4;Neoplastic transformation inhibitor protein;Nuclear antigen H731-like;Protein 197/15a;PDCD4;H731; Pdcd4|D19Ucl, D19Ucla1, MA-, Ma3, T, Tis|programmed cell death 4|programmed cell death protein 4|protein MA-3|topoisomerase-inhibitor suppressed protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDCD4, check out the PDCD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDCD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDCD4 Antibody Picoband® (A01105)
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Customer Q&As
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1 Customer Q&As for Anti-PDCD4 Antibody Picoband®
Question
We are currently using anti-PDCD4 antibody A01105 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
The anti-PDCD4 antibody (A01105) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-07