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Product Info Summary
| SKU: | A05752 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF |
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Product info
Product Name
Anti-CPI17a(Ab-38) PPP1R14A Antibody
View all CPI17 alpha Antibodies
SKU/Catalog Number
A05752
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CPI17a(Ab-38) PPP1R14A Antibody (Catalog # A05752). Tested in IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CPI17a(Ab-38) PPP1R14A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05752)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.36~40 (R-V-T-V-K) derived from Human CPI17a.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05752 is reactive to PPP1R14A in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
16693 MW
Background of CPI17 alpha
Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Hagerty L, et al. (2007) J Biol Chem. 2007; 282(7):4884-93
Seok YM, et al. (2008) J Pharmacol Exp Ther.
Niiro N et al. (2003) Biochem J. 2003 ;369(Pt 1):117-28
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05752 is guaranteed for IF Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 17kd
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Immunofluorescence staining of methanol-fixed Hela cells using CPI17a(Ab-38) antibody A05752.
Protein Target Info & Infographic
Gene/Protein Information For PPP1R14A (Source: Uniprot.org, NCBI)
Gene Name
PPP1R14A
Full Name
Protein phosphatase 1 regulatory subunit 14A
Weight
16693 MW
Superfamily
PP1 inhibitor family
Alternative Names
Protein phosphatase 1 regulatory subunit 14A;17 kDa PKC-potentiated inhibitory protein of PP1;Protein kinase C-potentiated inhibitor protein of 17 kDa;CPI-17;PPP1R14A;CPI17, PPP1INL; Ppp1r14a|1110001M11Rik, CPI-17, Cpi, Cpi17|protein phosphatase 1, regulatory inhibitor subunit 14A|protein phosphatase 1 regulatory subunit 14A|17 kDa PKC-potentiated inhibitory protein of PP1|protein kinase C-potentiated inhibitor protein of 17 kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1R14A, check out the PPP1R14A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP1R14A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CPI17a(Ab-38) PPP1R14A Antibody (A05752)
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No publications found for A05752
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CPI17a(Ab-38) PPP1R14A Antibody
Question
I am interested in to test anti-CPI17a(Ab-38) antibody A05752 on rat smooth muscle for research purposes, then I may be interested in using anti-CPI17a(Ab-38) antibody A05752 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The products we sell, including anti-CPI17a(Ab-38) antibody A05752, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-03
Question
My question regarding product A05752, anti-CPI17a(Ab-38) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05752 anti-CPI17a(Ab-38) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-22
Question
Is a blocking peptide available for product anti-CPI17a(Ab-38) antibody (A05752)?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
We do provide the blocking peptide for product anti-CPI17a(Ab-38) antibody (A05752). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-02
Question
Does anti-CPI17a(Ab-38) antibody A05752 work on bovine IF with liver?
T. Krishna
Verified customer
Asked: 2017-09-13
Answer
Our lab technicians have not validated anti-CPI17a(Ab-38) antibody A05752 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CPI17a(Ab-38) antibody A05752 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for smooth muscle using anti-CPI17a(Ab-38) antibody A05752. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-29
Question
I see that the anti-CPI17a(Ab-38) antibody A05752 works with IF, what is the protocol used to produce the result images on the product page?
J. Huang
Verified customer
Asked: 2015-07-22
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-07-22
Question
Is this A05752 anti-CPI17a(Ab-38) antibody reactive to the isotypes of PPP1R14A?
F. Huang
Verified customer
Asked: 2013-04-10
Answer
The immunogen of A05752 anti-CPI17a(Ab-38) antibody is Peptide sequence around aa.36~40 (R-V-T-V-K) derived from Human CPI17a. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-04-10
