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Product Info Summary
| SKU: | M01033-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Paxillin PXN Rabbit Monoclonal Antibody, Clone#RM256
SKU/Catalog Number
M01033-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Paxillin PXN Rabbit Monoclonal Antibody, Clone#RM256 (Catalog # M01033-1). Tested in WB, ICC, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Paxillin PXN Rabbit Monoclonal Antibody, Clone#RM256 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01033-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM256
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of human Paxillin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Paxillin. This antibody may also react to mouse or rat Paxillin, as predicted by immunogen homology.
Reactive Species
M01033-1 is reactive to PXN in Human
Reconstitution
Calculated molecular weight
64505 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01033-1 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry (ICC): 1:1000-1:2000 dilution
WB: 1:1000-1:2000 dilution
Immunohistochemistry (IHC): 1:1000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of HeLa cell lysate, using anti-Paxillin rabbit monoclonal Antibody Clone RM256 at a 1:1000 dilution.
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Figure 2. ICC result
Immunocytochemical staining of HeLa cells, using anti-Paxillin rabbit monoclonal Antibody Clone RM256 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue).
Protein Target Info & Infographic
Gene/Protein Information For PXN (Source: Uniprot.org, NCBI)
Gene Name
PXN
Full Name
Paxillin
Weight
64505 MW
Superfamily
paxillin family
Alternative Names
Paxillin;PXN; PXN paxillin paxillin|testicular tissue protein Li 134
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PXN, check out the PXN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PXN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Paxillin PXN Rabbit Monoclonal Antibody, Clone#RM256 (M01033-1)
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No publications found for M01033-1
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Customer Q&As
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15 Customer Q&As for Anti-Paxillin PXN Rabbit Monoclonal Antibody, Clone#RM256
Question
Is this M01033-1 anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 reactive to the isotypes of PXN?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The immunogen of M01033-1 anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 is A peptide corresponding to the N-terminus of human Paxillin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-17
Question
Would M01033-1 anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
You can see on the product datasheet, M01033-1 anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-06
Question
We have observed staining in human liver. Are there any suggestions? Is anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
From what I have seen in literature liver does express PXN. From what I have seen in Uniprot.org, PXN is expressed in fundus of stomach, placenta, fetal kidney, brain testis, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039
Boster Scientific Support
Answered: 2019-08-22
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fundus of stomach using anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 M01033-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-25
Question
I was wanting to use your anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 for WB for mouse fundus of stomach on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse fundus of stomach identification?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
You can see on the product datasheet, M01033-1 anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse fundus of stomach in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-27
Question
Is a blocking peptide available for product anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 (M01033-1)?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
We do provide the blocking peptide for product anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 (M01033-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-13
Question
Does anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 M01033-1 work for WB with fundus of stomach?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
According to the expression profile of fundus of stomach, PXN is highly expressed in fundus of stomach. So, it is likely that anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 M01033-1 will work for WB with fundus of stomach.
Boster Scientific Support
Answered: 2019-05-23
Question
I am interested in to test anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 M01033-1 on mouse fundus of stomach for research purposes, then I may be interested in using anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 M01033-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
The products we sell, including anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 M01033-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-22
Question
We are currently using anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 M01033-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
The anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 (M01033-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-25
Question
I see that the anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 M01033-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-02-16
Question
See below the WB image, lot number and protocol we used for fundus of stomach using anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 M01033-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-28
Question
I am interested in using your anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 for ptk6 regulates rho gtpases studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
A. Mitchell
Verified customer
Asked: 2016-08-10
Answer
Thank you for your inquiry. This M01033-1 anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 is validated on hela cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-08-10
Question
Is there a BSA free version of anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 M01033-1 available?
G. Mitchell
Verified customer
Asked: 2015-06-30
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256 M01033-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-06-30
Question
Can you help my question with product M01033-1, anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Singh
Verified customer
Asked: 2015-03-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01033-1 anti-Paxillin Rabbit Monoclonal antibody, Clone#RM256, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-03-04
Question
My colleagues were satisfied with the WB result of your anti-Paxillin Rabbit Monoclonal antibody, Clone
K. Li
Verified customer
Asked: 2015-02-26
Answer
According to literature, fetal kidney does express PXN. Generally PXN expresses in cytoplasm, cytoskeleton. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039
Boster Scientific Support
Answered: 2015-02-26
