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Product Info Summary
| SKU: | M00845 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Chicken, Human, Mouse, Rat, Zebrafish |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-PAX7 (Rhabdomyosarcoma Marker) Monoclonal Antibody
SKU/Catalog Number
M00845
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-PAX7 (Rhabdomyosarcoma Marker) Monoclonal Antibody (Catalog # M00845). Tested in IF, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat, Chicken, Zebrafish.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-PAX7 (Rhabdomyosarcoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00845)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: PAX7/497
Isotype
IgG1, kappa
Immunogen
Recombinant fragment (aa301-505) of human PAX7 protein (exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M00845 is reactive to PAX7 in Chicken, Human, Mouse, Rat, Zebrafish
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
55119 MW
Background of PAX7
The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00845 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Western Blot of HeLa cell lysate using Anti-PAX7 Mouse Monoclonal Antibody (PAX7/497).
Protein Target Info & Infographic
Gene/Protein Information For PAX7 (Source: Uniprot.org, NCBI)
Gene Name
PAX7
Full Name
Paired box protein Pax-7
Weight
55119 MW
Superfamily
paired homeobox family
Alternative Names
Paired box protein Pax-7;HuP1;PAX7;HUP1; PAX7 HUP1, MYOSCOB, RMS2, PAX7 paired box 7 paired box protein Pax-7|PAX7 transcriptional factor|paired box homeotic gene 7|paired domain gene 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAX7, check out the PAX7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAX7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAX7 (Rhabdomyosarcoma Marker) Monoclonal Antibody (M00845)
Hello CJ!
M00845 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li Li,Xiao Zhang,Hailong Yang et al.miR-193b-3p Promotes Proliferation of Goat Myoblast Through Directly Targeting IGF2BP1 and Activating Its Transcription,23 October 2020,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-95708/v1]
Species: Goat
M00845 usage in article: APP:IF, SAMPLE:MUSCS, DILUTION:1:100
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