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Product Info Summary
| SKU: | M00845-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-PAX7 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00845-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PAX7 Rabbit Monoclonal Antibody catalog # M00845-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAX7 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00845-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HGH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAX7
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00845-1 is reactive to PAX7 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
55119 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00845-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
FC 1:100
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of PAX7 expression in HeLa lysate.
Protein Target Info & Infographic
Gene/Protein Information For PAX7 (Source: Uniprot.org, NCBI)
Gene Name
PAX7
Full Name
Paired box protein Pax-7
Weight
55119 MW
Superfamily
paired homeobox family
Alternative Names
Paired box protein Pax-7;HuP1;PAX7;HUP1; PAX7 HUP1, MYOSCOB, RMS2, PAX7 paired box 7 paired box protein Pax-7|PAX7 transcriptional factor|paired box homeotic gene 7|paired domain gene 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAX7, check out the PAX7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAX7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAX7 Rabbit Monoclonal Antibody (M00845-1)
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No publications found for M00845-1
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Customer Q&As
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5 Customer Q&As for Anti-PAX7 Rabbit Monoclonal Antibody
Question
My question regarding product M00845-1, anti-PAX7 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00845-1 anti-PAX7 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-16
Question
Is this M00845-1 anti-PAX7 Rabbit Monoclonal antibody reactive to the isotypes of PAX7?
G. Parker
Verified customer
Asked: 2019-08-27
Answer
The immunogen of M00845-1 anti-PAX7 Rabbit Monoclonal antibody is A synthesized peptide derived from human PAX7 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-27
Question
We are currently using anti-PAX7 Rabbit Monoclonal antibody M00845-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
The anti-PAX7 Rabbit Monoclonal antibody (M00845-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-19
Question
Would M00845-1 anti-PAX7 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Miller
Verified customer
Asked: 2019-04-15
Answer
As indicated on the product datasheet, M00845-1 anti-PAX7 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-15
Question
I see that the anti-PAX7 Rabbit Monoclonal antibody M00845-1 works with WB, what is the protocol used to produce the result images on the product page?
D. Taylor
Verified customer
Asked: 2017-04-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-04-25
