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Product Info Summary
| SKU: | M01288 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PAX2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01288
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PAX2 Rabbit Monoclonal Antibody catalog # M01288. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAX2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01288)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HGB-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAX2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01288 is reactive to PAX2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
130 kDa
Calculated molecular weight
44706 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01288 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
Positive Control
WB: Human fetal kidney
Validation Images & Assay Conditions
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Western blot analysis of PAX2 expression in Human fetal kidney lysate.
Protein Target Info & Infographic
Gene/Protein Information For PAX2 (Source: Uniprot.org, NCBI)
Gene Name
PAX2
Full Name
Paired box protein Pax-2
Weight
44706 MW
Alternative Names
Paired box protein Pax-2;PAX2; PAX2 FSGS7, PAPRS paired box 2 paired box protein Pax-2|paired box homeotic gene 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAX2, check out the PAX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAX2 Rabbit Monoclonal Antibody (M01288)
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No publications found for M01288
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PAX2 Rabbit Monoclonal Antibody
Question
Is this M01288 anti-PAX2 Rabbit Monoclonal antibody reactive to the isotypes of PAX2?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
The immunogen of M01288 anti-PAX2 Rabbit Monoclonal antibody is A synthesized peptide derived from human PAX2 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-20
Question
Would anti-PAX2 Rabbit Monoclonal antibody M01288 work on goat WB with kidney?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
Our lab technicians have not tested anti-PAX2 Rabbit Monoclonal antibody M01288 on goat. You can run a BLAST between goat and the immunogen sequence of anti-PAX2 Rabbit Monoclonal antibody M01288 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-18
Question
My question regarding product M01288, anti-PAX2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01288 anti-PAX2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-28
Question
I was wanting to use to test anti-PAX2 Rabbit Monoclonal antibody M01288 on human cortex of kidney for research purposes, then I may be interested in using anti-PAX2 Rabbit Monoclonal antibody M01288 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
The products we sell, including anti-PAX2 Rabbit Monoclonal antibody M01288, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-20
Question
I see that the anti-PAX2 Rabbit Monoclonal antibody M01288 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-07
Question
Our team were well pleased with the WB result of your anti-PAX2 Rabbit Monoclonal antibody. However we have been able to see positive staining in leukemic t-cell nucleus. using this antibody. Is that expected? Could you tell me where is PAX2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
From literature, leukemic t-cell does express PAX2. Generally PAX2 expresses in nucleus. Regarding which tissues have PAX2 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 1378753
Kidney cortex, Pubmed ID: 7819127, 8661132
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2019-11-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cortex of kidney using anti-PAX2 Rabbit Monoclonal antibody M01288. Let me know if you need anything else.
Z. Baker
Verified customer
Asked: 2019-11-15
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-15
Question
Will anti-PAX2 Rabbit Monoclonal antibody M01288 work for WB with cortex of kidney?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
According to the expression profile of cortex of kidney, PAX2 is highly expressed in cortex of kidney. So, it is likely that anti-PAX2 Rabbit Monoclonal antibody M01288 will work for WB with cortex of kidney.
Boster Scientific Support
Answered: 2019-09-12
Question
I was wanting to use your anti-PAX2 Rabbit Monoclonal antibody for WB for human cortex of kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cortex of kidney identification?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
As indicated on the product datasheet, M01288 anti-PAX2 Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cortex of kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-22
Question
We have been able to see staining in human kidney cortex. Do you have any suggestions? Is anti-PAX2 Rabbit Monoclonal antibody supposed to stain kidney cortex positively?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
From literature kidney cortex does express PAX2. From Uniprot.org, PAX2 is expressed in cortex of kidney, kidney, kidney cortex, leukemic t-cell, among other tissues. Regarding which tissues have PAX2 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 1378753
Kidney cortex, Pubmed ID: 7819127, 8661132
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2018-07-25
Question
Would M01288 anti-PAX2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Taylor
Verified customer
Asked: 2018-03-13
Answer
It shows on the product datasheet, M01288 anti-PAX2 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-13
Question
We are currently using anti-PAX2 Rabbit Monoclonal antibody M01288 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
The anti-PAX2 Rabbit Monoclonal antibody (M01288) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-26
Question
See attached the WB image, lot number and protocol we used for cortex of kidney using anti-PAX2 Rabbit Monoclonal antibody M01288. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-30
Question
Do you have a BSA free version of anti-PAX2 Rabbit Monoclonal antibody M01288 available?
R. Miller
Verified customer
Asked: 2016-05-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PAX2 Rabbit Monoclonal antibody M01288 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-05-24
Question
Is a blocking peptide available for product anti-PAX2 Rabbit Monoclonal antibody (M01288)?
S. Edwards
Verified customer
Asked: 2015-07-28
Answer
We do provide the blocking peptide for product anti-PAX2 Rabbit Monoclonal antibody (M01288). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-07-28
