Anti-Pax2 Rabbit Monoclonal Antibody

PAX2 antibody

Boster Bio Anti-Pax2 Rabbit Monoclonal Antibody catalog # M01288-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: M01288-1
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IHC, WB

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Product Name

Anti-Pax2 Rabbit Monoclonal Antibody

View all PAX2 Antibodies

SKU/Catalog Number

M01288-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Pax2 Rabbit Monoclonal Antibody catalog # M01288-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Pax2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01288-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BDA-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Pax2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01288-1 is reactive to PAX2 in Human, Mouse

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

130 kDa

Calculated molecular weight

44706 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M01288-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50

Positive Control

WB: Human fetal kidney
IHC: human colon tissue

Validation Images & Assay Conditions

Gene/Protein Information For PAX2 (Source: Uniprot.org, NCBI)

Gene Name

PAX2

Full Name

Paired box protein Pax-2

Weight

44706 MW

Alternative Names

Paired box protein Pax-2;PAX2; PAX2 FSGS7, PAPRS paired box 2 paired box protein Pax-2|paired box homeotic gene 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PAX2, check out the PAX2 Infographic

PAX2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PAX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Pax2 Rabbit Monoclonal Antibody

Question

We are currently using anti-Pax2 Rabbit Monoclonal antibody M01288-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?

O. Walker

Verified customer

Asked: 2019-12-03

Answer

The anti-Pax2 Rabbit Monoclonal antibody (M01288-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-03

Question

We were well pleased with the WB result of your anti-Pax2 Rabbit Monoclonal antibody. However we have observed positive staining in cortex of kidney nucleus. using this antibody. Is that expected? Could you tell me where is PAX2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-03-22

Answer

Based on literature, cortex of kidney does express PAX2. Generally PAX2 expresses in nucleus. Regarding which tissues have PAX2 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 1378753
Kidney cortex, Pubmed ID: 7819127, 8661132
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2019-03-22

Question

We purchased anti-Pax2 Rabbit Monoclonal antibody for WB on kidney last year. I am using mouse, and We intend to use the antibody for IHC next. My lab would like examining kidney as well as kidney cortex in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

T. Edwards

Verified customer

Asked: 2018-12-05

Answer

I looked at the website and datasheets of our anti-Pax2 Rabbit Monoclonal antibody and it appears that M01288-1 has been validated on mouse in both WB and IHC. Thus M01288-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-12-05

Question

We have been able to see staining in mouse cortex of kidney. Are there any suggestions? Is anti-Pax2 Rabbit Monoclonal antibody supposed to stain cortex of kidney positively?

K. Evans

Verified customer

Asked: 2013-07-23

Answer

From what I have seen in literature cortex of kidney does express PAX2. From what I have seen in Uniprot.org, PAX2 is expressed in cortex of kidney, kidney, kidney cortex, leukemic t-cell, among other tissues. Regarding which tissues have PAX2 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 1378753
Kidney cortex, Pubmed ID: 7819127, 8661132
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2013-07-23

Order DetailsPrice
M01288-1

100μL

$370
M01288-1-30ul

30μL sample

$99
M01288-1-carrier-free-1ml

Carrier Free

$2590

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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