Product Info Summary
SKU: | M01288-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Pax2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01288-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Pax2 Rabbit Monoclonal Antibody catalog # M01288-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pax2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01288-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BDA-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Pax2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01288-1 is reactive to PAX2 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
130 kDa
Calculated molecular weight
44706 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01288-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50
Positive Control
WB: Human fetal kidney
IHC: human colon tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Pax2 expression in Human fetal kidney lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon using Pax2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For PAX2 (Source: Uniprot.org, NCBI)
Gene Name
PAX2
Full Name
Paired box protein Pax-2
Weight
44706 MW
Alternative Names
Paired box protein Pax-2;PAX2; PAX2 FSGS7, PAPRS paired box 2 paired box protein Pax-2|paired box homeotic gene 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAX2, check out the PAX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Pax2 Rabbit Monoclonal Antibody (M01288-1)
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No publications found for M01288-1
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Customer Q&As
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4 Customer Q&As for Anti-Pax2 Rabbit Monoclonal Antibody
Question
We are currently using anti-Pax2 Rabbit Monoclonal antibody M01288-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
O. Walker
Verified customer
Asked: 2019-12-03
Answer
The anti-Pax2 Rabbit Monoclonal antibody (M01288-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-03
Question
We were well pleased with the WB result of your anti-Pax2 Rabbit Monoclonal antibody. However we have observed positive staining in cortex of kidney nucleus. using this antibody. Is that expected? Could you tell me where is PAX2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
Based on literature, cortex of kidney does express PAX2. Generally PAX2 expresses in nucleus. Regarding which tissues have PAX2 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 1378753
Kidney cortex, Pubmed ID: 7819127, 8661132
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2019-03-22
Question
We purchased anti-Pax2 Rabbit Monoclonal antibody for WB on kidney last year. I am using mouse, and We intend to use the antibody for IHC next. My lab would like examining kidney as well as kidney cortex in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
T. Edwards
Verified customer
Asked: 2018-12-05
Answer
I looked at the website and datasheets of our anti-Pax2 Rabbit Monoclonal antibody and it appears that M01288-1 has been validated on mouse in both WB and IHC. Thus M01288-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-05
Question
We have been able to see staining in mouse cortex of kidney. Are there any suggestions? Is anti-Pax2 Rabbit Monoclonal antibody supposed to stain cortex of kidney positively?
K. Evans
Verified customer
Asked: 2013-07-23
Answer
From what I have seen in literature cortex of kidney does express PAX2. From what I have seen in Uniprot.org, PAX2 is expressed in cortex of kidney, kidney, kidney cortex, leukemic t-cell, among other tissues. Regarding which tissues have PAX2 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 1378753
Kidney cortex, Pubmed ID: 7819127, 8661132
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2013-07-23