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Product Info Summary
| SKU: | M04041-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Chicken |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Parvalbumin Antibody
View all Parvalbumin Antibodies
SKU/Catalog Number
M04041-2
Size
100ul
Description
Boster Bio Anti-Parvalbumin Antibody catalog # M04041-2. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Parvalbumin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04041-2)
Host
Chicken
Contents
Purified IgY preparation with 5mM NaN3 at 20-30 mg/mL
Clonality
Polyclonal
Immunogen
Full-length recombinant human protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04041-2 is reactive to PVALB in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
12059 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04041-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000 IF/ICC or IHC: 1:1,000-1:5,000
Validation Images & Assay Conditions
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Western blot analysis of skeletal muscle lysates and His-tagged recombinant human proteins using chicken pAb to parvalbumin, M04041-2, dilution 1:2,000, in green: [1] protein standard (red), [2] mouse muscle, [3] recombinant parvalbumin, [4] recombinant calretinin and [5] recombinant calbindin. Band at 12kDa in muscle lysate is native parvalbumin and 18kDa band in the next lane is His-tagged recombinant parvalbumin. The M04041-2 antibody binds parvalbumin strongly but is not cross-reactive with the closely related calcium binding proteins calretinin or calbindin.
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Immunofluorescent analysis of a rat cerebellum section stained with chicken pAb to parvalbumin, M04041-2, dilution 1:2,000, in green, and costained with mouse mAb to GFAP, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free floating sections were stained with above antibodies. The M04041-2 antibody labels the perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astrocytes.
Protein Target Info & Infographic
Gene/Protein Information For PVALB (Source: Uniprot.org, NCBI)
Gene Name
PVALB
Full Name
Parvalbumin alpha
Weight
12059 MW
Superfamily
parvalbumin family
Alternative Names
Parvalbumin alpha;PVALB; PVALB D22S749 parvalbumin parvalbumin alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PVALB, check out the PVALB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PVALB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Parvalbumin Antibody (M04041-2)
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1 Customer Q&As for Anti-Parvalbumin Antibody
Question
We are currently using anti-Parvalbumin antibody M04041-2 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-12
Answer
The anti-Parvalbumin antibody (M04041-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-12
