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Product Info Summary
| SKU: | PA2129 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-P2X purinoceptor 7 P2RX7 Antibody Picoband®
View all P2X7/P2RX7 Antibodies
SKU/Catalog Number
PA2129
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-P2X purinoceptor 7 P2RX7 Antibody catalog # PA2129. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P2X purinoceptor 7 P2RX7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2129)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat P2RX7, different from the related mouse sequence by one amino acid, and from the related human sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2129 is reactive to P2rx7 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
68392 MW
Background of P2X7/P2RX7
P2X purinoceptor 7, also called P2X7, is a protein that in humans is encoded by the P2RX7 gene. The product of this gene belongs to the family of purnoceptors for ATP. P2RX7 was mapped to chromosome 12q24 by fluorescence in situ hybridization. It is a receptor for ATP that acts as a ligand-gated ion channel. This gene is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. It could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2129 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: U87 Cell
Validation Images & Assay Conditions
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Anti-P2RX7 antibody, PA2129, Western blotting
WB: U87 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For P2rx7 (Source: Uniprot.org, NCBI)
Gene Name
P2rx7
Full Name
P2X purinoceptor 7
Weight
68392 MW
Superfamily
P2X receptor family
Alternative Names
P2X purinoceptor 7;P2X7;ATP receptor;P2Z receptor;Purinergic receptor;P2rx7; P2RX7 P2X7 purinergic receptor P2X 7 P2X purinoceptor 7|ATP receptor|P2X7 receptor|P2Z receptor|purinergic receptor P2X, ligand gated ion channel, 7|purinergic receptor P2X7 variant A|purnergic receptor P2X 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on P2rx7, check out the P2rx7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for P2rx7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-P2X purinoceptor 7 P2RX7 Antibody Picoband® (PA2129)
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No publications found for PA2129
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Customer Q&As
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6 Customer Q&As for Anti-P2X purinoceptor 7 P2RX7 Antibody Picoband®
Question
We are currently using anti-P2RX7 antibody PA2129 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The anti-P2RX7 antibody (PA2129) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-24
Question
My question regarding product PA2129, anti-P2RX7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2129 anti-P2RX7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-12
Question
I see that the anti-P2RX7 antibody PA2129 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-02
Question
I was wanting to use to test anti-P2RX7 antibody PA2129 on mouse brain skin for research purposes, then I may be interested in using anti-P2RX7 antibody PA2129 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
The products we sell, including anti-P2RX7 antibody PA2129, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain skin using anti-P2RX7 antibody PA2129. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-25
Question
I have attached the WB image, lot number and protocol we used for brain skin using anti-P2RX7 antibody PA2129. Please let me know if you require anything else.
K. Krishna
Verified customer
Asked: 2013-01-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-04
