Anti-PARP10 Antibody Picoband™

Product Name

Anti-PARP10 Antibody Picoband™

View all PARP10 Antibodies

SKU/Catalog Number

A08514-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PARP10 Antibody Picoband™ catalog # A08514-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PARP10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08514-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human PARP10 recombinant protein (Position: E14-E251). Human PARP10 shares 76.5% amino acid (aa) sequence identity with mouse PARP10.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08514-2 is reactive to PARP10 in Human

Applications

A08514-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

140 kDa

Calculated molecular weight

52588 MW

Background of PARP10

Poly [ADP-ribose] polymerase 10 is an enzyme that in humans is encoded by the PARP10 gene. Poly(ADP-ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP-ribose to histones. PARPs can also function as transcriptional cofactors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PARP10 using anti-PARP10 antibody (A08514-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP10 antigen affinity purified polyclonal antibody (Catalog # A08514-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP10 at approximately 140 kDa. The expected band size for PARP10 is at 110 kDa.

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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-PARP10 antibody (A08514-2).
Overlay histogram showing MCF-7 cells stained with A08514-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARP10 Antibody (A08514-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PARP10 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.4.2.30; FLJ14464; PARP-10; poly (ADP-ribose) polymerase family, member 10; poly [ADP-ribose] polymerase 10 PARP10 ARTD10 poly(ADP-ribose) polymerase family member 10 protein mono-ADP-ribosyltransferase PARP10|ADP-ribosyltransferase diphtheria toxin-like 10|poly [ADP-ribose] polymerase 10

For more info on PARP10, check out the PARP10 Infographic

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In this infographic, you will see the following information for PARP10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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