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Product Info Summary
| SKU: | A14355-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PAQR6 Antibody Picoband®
SKU/Catalog Number
A14355-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PAQR6 Antibody Picoband® catalog # A14355-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PAQR6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14355-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PAQR6 recombinant protein (Position: M1-A292).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A14355-1 is reactive to PAQR6 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
64099 MW
Background of Paqr6
Membrane progesterone receptor delta (mPRδ), or progestin and adipoQ receptor 6 (PAQR6), is a protein that in humans is encoded by the PAQR6 gene. Predicted to enable signaling receptor activity. Predicted to be located in plasma membrane. Predicted to be integral component of membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14355-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PAQR6 using anti-PAQR6 antibody (A14355-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAQR6 antigen affinity purified polyclonal antibody (Catalog # A14355-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAQR6 at approximately 38 kDa. The expected band size for PAQR6 is at 38 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PAQR6 (Source: Uniprot.org, NCBI)
Gene Name
PAQR6
Full Name
Membrane progestin receptor delta
Weight
64099 MW
Superfamily
ADIPOR family
Alternative Names
RNA-binding protein 47;RNA-binding motif protein 47;RBM47;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAQR6, check out the PAQR6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAQR6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAQR6 Antibody Picoband® (A14355-1)
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