Anti-Palladin/PALLD Antibody Picoband™

Product Name

Anti-Palladin/PALLD Antibody Picoband™

View all Palladin Antibodies

SKU/Catalog Number

A04005-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Palladin/PALLD Antibody Picoband™ catalog # A04005-3. Tested in WB, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Palladin/PALLD Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04005-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human Palladin/PALLD recombinant protein (Position: H89-L1383). Human PALLD shares 85.5% amino acid (aa) sequence identity with mouse PALLD.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A04005-3 is reactive to PALLD in Human

Applications

A04005-3 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

95 kDa

Calculated molecular weight

52588 MW

Background of Palladin

Palladin is a protein that in humans is encoded by the PALLD gene. This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments, and it is involved in the control of cell shape, adhesion, and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1, and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Palladin/PALLD using anti-Palladin/PALLD antibody (A04005-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Palladin/PALLD antigen affinity purified polyclonal antibody (Catalog # A04005-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Palladin/PALLD at approximately 95 kDa. The expected band size for Palladin/PALLD is at 151 kDa.

Gene/Protein Information For PALLD (Source: Uniprot.org, NCBI)

Alternative Names

CGI151; CGI-151; FLJ22190; FLJ38193; KIAA0992FLJ39139; MYN; myoneurin; Palladin; palladin, cytoskeletal associated protein; PALLD; PNCA1; Sarcoma antigen NY-SAR-77; SIH002; SIH002FLJ61376 PALLD CGI-151, CGI151, MYN, PNCA1, SIH002 palladin, cytoskeletal associated protein palladin|myoneurin|sarcoma antigen NY-SAR-77

For more info on PALLD, check out the PALLD Infographic

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In this infographic, you will see the following information for PALLD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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