Click image to see more details
Product Info Summary
| SKU: | M03711 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Cow, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IP, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PAG1 Purified Monoclonal Antibody
SKU/Catalog Number
M03711
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PAG1 Purified Monoclonal Antibody (Catalog# M03711). Tested in IP, WB, IHC-P application(s). This antibody reacts with Rat, Cow, Human, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-PAG1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03711)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
PAG-C1
Isotype
Mouse IgG2b
Immunogen
C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH. The antibody PAG-C1 recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M03711 is reactive to PAG1 in Cow, Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
46981 MW
Background of PAG1
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03711 is guaranteed for IP, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): Positive tissue: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1.
Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of PAG1 using Anti-PAG1 Purified Monoclonal Antibody (M03711).
Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-PAG1 Purified Monoclonal Antibody M03711
Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line.Western blot was immunostained with anti-human PAG (MEM-255; cat. no. 11-347-C100).
Note: PAG/Cbp is a 46 kDa adaptor protein
Protein Target Info & Infographic
Gene/Protein Information For PAG1 (Source: Uniprot.org, NCBI)
Gene Name
PAG1
Full Name
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
Weight
46981 MW
Alternative Names
CBP, PAG PAG1 CBP, PAG phosphoprotein membrane anchor with glycosphingolipid microdomains 1 phosphoprotein associated with glycosphingolipid-enriched microdomains 1|Csk-binding protein|phosphoprotein associated with glycosphingolipid microdomains 1|transmembrane adapter protein PAG|transmembrane adaptor protein PAG|transmembrane phosphoprotein Cbp
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAG1, check out the PAG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAG1 Purified Monoclonal Antibody (M03711)
Hello CJ!
No publications found for M03711
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PAG1 Purified Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PAG1 Purified Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
