Anti-PAFAH1B2 Antibody Picoband™

Product Name

Anti-PAFAH1B2 Antibody Picoband™

View all PAFAH1B2 Antibodies

SKU/Catalog Number

A07067

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PAFAH1B2 Antibody Picoband™ catalog # A09276-3. Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PAFAH1B2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07067)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human PAFAH1B2 recombinant protein (Position: D5-D190). Human PAFAH1B2 shares 99.5% amino acid (aa) sequence identity with both mouse and rat PAFAH1B2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A07067 is reactive to PAFAH1B2 in Human, Mouse, Rat

Applications

A07067 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

52588 MW

Background of PAFAH1B2

Platelet-activating factor acetylhydrolase IB subunit beta is an enzyme that in humans is encoded by the PAFAH1B2 gene. Platelet-activating factor acetylhydrolase (PAFAH) inactivates platelet-activating factor (PAF) into acetate and LYSO-PAF. This gene encodes the beta subunit of PAFAH, the other subunits are alpha and gamma. Multiple alternatively spliced transcript variants have been described for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse
Immunohistochemistry, 2-5 µg/ml, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PAFAH1B2 using anti-PAFAH1B2 antibody (A07067).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH1B2 antigen affinity purified polyclonal antibody (Catalog # A07067) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAFAH1B2 at approximately 26 kDa. The expected band size for PAFAH1B2 is at 26 kDa.

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Figure 2. IHC analysis of PAFAH1B2 using anti-PAFAH1B2 antibody (A07067).
PAFAH1B2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAFAH1B2 Antibody (A07067) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of PAFAH1B2 using anti-PAFAH1B2 antibody (A07067).
PAFAH1B2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAFAH1B2 Antibody (A07067) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For PAFAH1B2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.1.1.47; intracellular platelet-activating factor acetylhydrolase alpha 2 subunit; PAF acetylhydrolase 30 kDa subunit; PAF-AH 30 kDa subunit; PAFAH subunit beta; PAF-AH subunit beta; PAF-AH1b alpha 2 subunit; PAFAHB; platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa); platelet-activating factor acetylhydrolase IB subunit beta; platelet-activating factor acetylhydrolase, isoform Ib, beta subunit 30kDa; platelet-activating factor acetylhydrolase, isoform Ib, subunit 2 (30kDa) PAFAH1B2 HEL-S-303 platelet activating factor acetylhydrolase 1b catalytic subunit 2 platelet-activating factor acetylhydrolase IB subunit alpha2|PAF acetylhydrolase 30 kDa subunit|PAF-AH 30 kDa subunit|PAF-AH subunit beta|PAF-AH1b alpha 2 subunit|PAFAH subunit beta|epididymis secretory protein Li 303|epididymis secretory sperm binding protein|intracellular platelet-activating factor acetylhydrolase alpha 2 subunit|platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa)|platelet-activating factor acetylhydrolase IB subunit beta

For more info on PAFAH1B2, check out the PAFAH1B2 Infographic

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In this infographic, you will see the following information for PAFAH1B2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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