Product Info Summary
SKU: | M01058-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse |
Host: | Mouse |
Application: | ELISA, WB |
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Product info
Product Name
Anti-p90RSK RPS6KA1 Monoclonal Antibody
SKU/Catalog Number
M01058-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-p90RSK RPS6KA1 Monoclonal Antibody catalog # M01058-1. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p90RSK RPS6KA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01058-1)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
6B9-D6-F8
Isotype
IgG
Immunogen
Purified recombinant human p90RSK protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01058-1 is reactive to RPS6KA1 in Human, Monkey, Mouse
Applications
M01058-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
82723 MW
Background of RSK1
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ELISA 1:10000-20000
Validation Images & Assay Conditions
![m01058 1 anti p90rsk rps6ka1 monoclonal antibody ip testing 2 m01058 1 anti p90rsk rps6ka1 monoclonal antibody ip testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m01058-1-anti-p90rsk-rps6ka1-monoclonal-antibody-ip-testing-2.jpg)
Click image to see more details
Figure 2. IP validation of RPS6KA1 using Anti-p90RSK RPS6KA1 Monoclonal Antibody (M01058-1).
Immunoprecipitation analysis of Hela cell lysates using p90RSK mouse mAb.![m01058 1 anti p90rsk rps6ka1 monoclonal antibody wb testing 1 m01058 1 anti p90rsk rps6ka1 monoclonal antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m01058-1-anti-p90rsk-rps6ka1-monoclonal-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-p90RSK RPS6KA1 Monoclonal Antibody M01058-1
Western blot detection of p90RSK in COS7Protein Target Info & Infographic
Gene/Protein Information For RPS6KA1 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KA1
Full Name
Ribosomal protein S6 kinase alpha-1
Weight
82723 MW
Superfamily
protein kinase superfamily
Alternative Names
90kD, polypeptide 1); EC 2.7.11; EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1; HU-1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPL1A; p90RSK1; p90S6K; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase, 90kD, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RPS6KA1; RSK; RSK1; RSK-1; RSK1p90-RSK 1; S6K-alpha 1; S6K-alpha-1 RPS6KA1 HU-1, MAPKAPK1, MAPKAPK1A, RSK, RSK1, p90Rsk ribosomal protein S6 kinase A1 ribosomal protein S6 kinase alpha-1|90 kDa ribosomal protein S6 kinase 1|MAP kinase-activated protein kinase 1a|MAPK-activated protein kinase 1a|MAPKAP kinase 1a|MAPKAPK-1a|RSK-1|S6K-alpha 1|dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)|p90-RSK 1|p90RSK1|p90S6K|ribosomal S6 kinase 1|ribosomal protein S6 kinase, 90kDa, polypeptide 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS6KA1, check out the RPS6KA1 Infographic
![RPS6KA1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS6KA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p90RSK RPS6KA1 Monoclonal Antibody (M01058-1)
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