Click image to see more details
Product Info Summary
| SKU: | A07956-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-p56Dok-2 (Ab-299) Antibody
SKU/Catalog Number
A07956-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-p56Dok-2 (Ab-299) Antibody (Catalog # A07956-2). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p56Dok-2 (Ab-299) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07956-2)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.297~301 (G-E-Y-A-V) derived from Human p56Dok-2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07956-2 is reactive to DOK2 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
45379 MW
Background of DOK2
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation
Feng Cong, et,al. (1999) Mol. Cell. Biol; 19: 8314 - 8325.
Serge Lemay, et,al. (2000) Mol. Cell. Biol; 20: 2743 - 2754.
Ute Schaeper, et,al.(2000) J. Cell Biol; 149: 1419.
Miyuki Honma, et,al. (2006) Genes Cells; 11: 143 - 151.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07956-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 56kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of DOK2 using anti-DOK2 antibody (A07956-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOK2 antigen affinity purified polyclonal antibody (Catalog # A07956-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for DOK2.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using p56Dok-2(Ab-299) Antibody #A07956-2.
Click image to see more details
Figure 2. IHC analysis of DOK2 using anti-DOK2 antibody (A07956-2).
DOK2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DOK2 Antibody (A07956-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For DOK2 (Source: Uniprot.org, NCBI)
Gene Name
DOK2
Full Name
Docking protein 2
Weight
45379 MW
Superfamily
DOK family
Alternative Names
Docking protein 2;Downstream of tyrosine kinase 2;p56 (dok-2);DOK2; DOK2 p56DOK, p56dok-2 docking protein 2 docking protein 2|docking protein 2, 56kD|docking protein 2, 56kDa|downstream of tyrosine kinase 2|p56(dok-2)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DOK2, check out the DOK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DOK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p56Dok-2 (Ab-299) Antibody (A07956-2)
Hello CJ!
No publications found for A07956-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-p56Dok-2 (Ab-299) Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-p56Dok-2 (Ab-299) Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
