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Product Info Summary
| SKU: | A07698 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-p50 Dynamitin DCTN2 Antibody
View all Dynactin Subunit 2/DCTN2/DCTN-50 Antibodies
SKU/Catalog Number
A07698
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-p50 Dynamitin DCTN2 Antibody (Catalog # A07698). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p50 Dynamitin DCTN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07698)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human p50 dynamitin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07698 is reactive to DCTN2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
44231 MW
Background of Dynactin Subunit 2/DCTN2/DCTN-50
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
Scott S. Grieshaber, J. Cell Sci., Sep 2003; 116: 3793.
Janis K. Burkhardt, J. Cell Biol., Oct 1997; 139: 469.
Karin A. Melkonian, J. Biol. Chem., Apr 2007; 10.1074.
Lili Yue, J. Biol. Chem., Jul 2000; 275: 23948.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07698 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HuvEc cells (Lane 2), using p50 Dynamitin antiobdy A07698. The lane on the left is treated with synthesized peptide.
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Western blot analysis of extracts from A549 cells, using p50 dynamitin antibody A07698.
Protein Target Info & Infographic
Gene/Protein Information For DCTN2 (Source: Uniprot.org, NCBI)
Gene Name
DCTN2
Full Name
Dynactin subunit 2
Weight
44231 MW
Superfamily
dynactin subunit 2 family
Alternative Names
Dynactin subunit 2;50 kDa dynein-associated polypeptide;Dynactin complex 50 kDa subunit;DCTN-50;p50 dynamitin;DCTN2;DCTN50; DCTN2 DCTN50, DYNAMITIN, HEL-S-77, RBP50 dynactin subunit 2 dynactin subunit 2|50 kDa dynein-associated polypeptide|dynactin 2 (p50)|dynactin complex 50 kDa subunit|epididymis secretory protein Li 77|epididymis secretory sperm binding protein|p50 dynamitin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DCTN2, check out the DCTN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DCTN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p50 Dynamitin DCTN2 Antibody (A07698)
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No publications found for A07698
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-p50 Dynamitin DCTN2 Antibody
Question
Is a blocking peptide available for product anti-p50 Dynamitin antibody (A07698)?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
We do provide the blocking peptide for product anti-p50 Dynamitin antibody (A07698). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-08
Question
Is this A07698 anti-p50 Dynamitin antibody reactive to the isotypes of DCTN2?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The immunogen of A07698 anti-p50 Dynamitin antibody is Synthesized peptide derived from human p50 dynamitin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-15
Question
My question regarding product A07698, anti-p50 Dynamitin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07698 anti-p50 Dynamitin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-03
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-p50 Dynamitin antibody A07698. Please let me know if you require anything else.
K. Edwards
Verified customer
Asked: 2018-07-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-09
Question
Would A07698 anti-p50 Dynamitin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
It shows on the product datasheet, A07698 anti-p50 Dynamitin antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-02
Question
Is there a BSA free version of anti-p50 Dynamitin antibody A07698 available?
Verified Customer
Verified customer
Asked: 2017-07-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p50 Dynamitin antibody A07698 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-24
Question
We are currently using anti-p50 Dynamitin antibody A07698 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
D. Yang
Verified customer
Asked: 2014-11-17
Answer
The anti-p50 Dynamitin antibody (A07698) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-11-17
