Anti-Myosin MYL9 Antibody

Product Name

Anti-Myosin MYL9 Antibody

View all MRCL3 Antibodies

SKU/Catalog Number

A06446

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Myosin MYL9 Antibody (Catalog # A06446). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Myosin MYL9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06446)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids aa 10-35 of human myosin light chain protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06446 is reactive to MYL12A in Mouse, Rat

Applications

A06446 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

19827 MW

Background of MRCL3

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:70,000
IHC: User optimized
IP: 1:100
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Western blot analysis of Myosin expression in vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane 2). Arrowheads indicate the detection of both mono-phosphorylated (upper) and unphosphorylated (lower) forms of the protein. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Myosin was detected using rabbit anti-Myosin Antigen Affinity purified polyclonal antibody (Catalog # A06446) at 1:600. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For MYL12A (Source: Uniprot.org, NCBI)

Alternative Names

MLCBMyosin RLC; MRCL3; MRLC3MLC-2B; MYL2B; myosin regulatory light chain 12A; Myosin regulatory light chain 2, nonsarcomeric; myosin regulatory light chain 3; myosin regulatory light chain MRCL3; Myosin regulatory light chain MRLC3; myosin, light chain 12A, regulatory, non-sarcomeric; myosin, light polypeptide, regulatory, non-sarcomeric (20kD); RLC MYL12A HEL-S-24, MLC-2B, MLCB, MRCL3, MRLC3, MYL2B myosin light chain 12A myosin regulatory light chain 12A|epididymis secretory protein Li 24|myosin RLC|myosin regulatory light chain 2, nonsarcomeric|myosin regulatory light chain 3|myosin regulatory light chain MRLC3|myosin, light chain 12A, regulatory, non-sarcomeric|myosin, light polypeptide, regulatory, non-sarcomeric (20kD)

For more info on MYL12A, check out the MYL12A Infographic

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In this infographic, you will see the following information for MYL12A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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