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Product Info Summary
| SKU: | M00176-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00176-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody catalog # M00176-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00176-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EGC-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p38 MAPK
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00176-1 is reactive to MAPK14 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
41, 38 kDa
Calculated molecular weight
41293 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00176-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human Jurkat whole cell, human SW620 whole cell, human CACO-2 whole cell, human Hela whole cell, rat heart tissue, rat lung tissue, mouse heart tissue, mouse lung tissue
IHC: human brain tissue, Human thyroid cancer tissue, Human prostate cancer tissue, Human astrocytoma tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of p38 MAPK using anti-p38 MAPK antibody (M00176-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p38 MAPK antigen affinity purified monoclonal antibody (Catalog # M00176-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p38 MAPK at approximately 41, 38 kDa. The expected band size for p38 MAPK is at 41 kDa.
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IHC analysis of p38 MAPK using anti-p38 MAPK antibody (M00176-1) on human brain.
p38 MAPK was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-p38 MAPK Antibody (M00176-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody at 1:300 dilution.
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IF analysis of immunocytochemical section of Hela cells using anti-p38 MAPK antibody (M00176-1)
p38 MAPK was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-p38 MAPK Antibody (M00176-1) overnight at 4 °
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For MAPK14 (Source: Uniprot.org, NCBI)
Gene Name
MAPK14
Full Name
Mitogen-activated protein kinase 14
Weight
41293 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase 14;MAP kinase 14;MAPK 14;2.7.11.24 ;Cytokine suppressive anti-inflammatory drug-binding protein;CSAID-binding protein;CSBP;MAP kinase MXI2;MAX-interacting protein 2;Mitogen-activated protein kinase p38 alpha;MAP kinase p38 alpha;Stress-activated protein kinase 2a;SAPK2a;MAPK14;CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A; MAPK14 CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA mitogen-activated protein kinase 14 mitogen-activated protein kinase 14|CSAID-binding protein|MAP kinase 14|MAP kinase Mxi2|MAP kinase p38 alpha|MAX-interacting protein 2|cytokine suppressive anti-inflammatory drug binding protein|mitogen-activated protein kinase p38 alpha|p38 MAP kinase|p38 mitogen activated protein kinase|p38alpha Exip|stress-activated protein kinase 2A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK14, check out the MAPK14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody (M00176-1)
Hello CJ!
M00176-1 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wei X,Zhu Q,Liu N,Xu L,Wei S,Fan Z,Sun C,Zhao Y,Qiao M,Wu J,Hu D,Wang Y,Sun P.Neuroprotective Effects and Mechanisms of Zhenlong Xingnao Capsule in In Vivo and In Vitro Models of Hypoxia.Front Pharmacol.2019 Sep 26;10:1096.doi:10.3389/fphar.2019.01096.PMID:31611791;PMCID:PMC6775503.
Species: Rat
M00176-1 usage in article: APP:IHC, SAMPLE:CEREBRAL TISSUE, DILUTION: NA
Ma G,Kimatu BM,Yang W,Pei F,Zhao L,Du H,Su A,Hu Q,Xiao H.Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential.J Food Sci.2020 Sep;85(9):2822-2831. doi:10.1111/1750-3841.15375.Epub 2020 Aug 14
Species: Mouse
M00176-1 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA
Vasopressin decreases neuronal apoptosis during cardiopulmonary resuscitation
Liao S, Xiao S, Chen H, Zhang M, Chen Z, Long Y, Gao L, He J, Ge Y, Yi W, Wu M, Li G, Zhou Y. Int J Oncol. 2017 Nov;51(5):1497-1507. doi: 10.3892/ijo.2017.4137. Epub 2017 Sep 27. The receptor for activated protein kinase C promotes cell growth, in...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody
Question
Would M00176-1 anti-p38 MAPK Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
It shows on the product datasheet, M00176-1 anti-p38 MAPK Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-21
Question
Is a blocking peptide available for product anti-p38 MAPK Rabbit Monoclonal antibody (M00176-1)?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We do provide the blocking peptide for product anti-p38 MAPK Rabbit Monoclonal antibody (M00176-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-25
Question
Do you have a BSA free version of anti-p38 MAPK Rabbit Monoclonal antibody M00176-1 available?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-p38 MAPK Rabbit Monoclonal antibody M00176-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-13
Question
Is this M00176-1 anti-p38 MAPK Rabbit Monoclonal antibody reactive to the isotypes of MAPK14?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
The immunogen of M00176-1 anti-p38 MAPK Rabbit Monoclonal antibody is A synthesized peptide derived from human p38 MAPK. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-27
Question
Will anti-p38 MAPK Rabbit Monoclonal antibody M00176-1 work for IHC with platelet?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
According to the expression profile of platelet, MAPK14 is highly expressed in platelet. So, it is likely that anti-p38 MAPK Rabbit Monoclonal antibody M00176-1 will work for IHC with platelet.
Boster Scientific Support
Answered: 2020-01-20
Question
Please see the WB image, lot number and protocol we used for platelet using anti-p38 MAPK Rabbit Monoclonal antibody M00176-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-25
Question
My team were well pleased with the WB result of your anti-p38 MAPK Rabbit Monoclonal antibody. However we have observed positive staining in renal cell carcinoma cytoplasm. using this antibody. Is that expected? Could you tell me where is MAPK14 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
From literature, renal cell carcinoma does express MAPK14. Generally MAPK14 expresses in cytoplasm. Regarding which tissues have MAPK14 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 10727080
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Hepatoma, Pubmed ID: 10943842
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 7696354, 24275569
Peripheral blood, Pubmed ID: 7997261
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801, 18088087
Renal cell carcinoma, Pubmed ID: 11866441
T-cell, Pubmed ID: 21586573
Boster Scientific Support
Answered: 2019-12-13
Question
We are interested in to test anti-p38 MAPK Rabbit Monoclonal antibody M00176-1 on human platelet for research purposes, then I may be interested in using anti-p38 MAPK Rabbit Monoclonal antibody M00176-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The products we sell, including anti-p38 MAPK Rabbit Monoclonal antibody M00176-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-20
Question
Can you help my question with product M00176-1, anti-p38 MAPK Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00176-1 anti-p38 MAPK Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-20
Question
Our lab used your anti-p38 MAPK Rabbit Monoclonal antibody for ICC on platelet in a previous experiment. I am using rat, and I plan to use the antibody for IF next. Our lab want to know about examining platelet as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
I have checked the website and datasheets of our anti-p38 MAPK Rabbit Monoclonal antibody and I see that M00176-1 has been validated on rat in both ICC and IF. Thus M00176-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-04
Question
We are currently using anti-p38 MAPK Rabbit Monoclonal antibody M00176-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
The anti-p38 MAPK Rabbit Monoclonal antibody (M00176-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-22
Question
I see that the anti-p38 MAPK Rabbit Monoclonal antibody M00176-1 works with IHC, what is the protocol used to produce the result images on the product page?
M. Huang
Verified customer
Asked: 2017-12-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-12-06
Question
My lab would like using your anti-p38 MAPK Rabbit Monoclonal antibody for vegfa-vegfr2 pathway studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-08-15
Answer
We appreciate your inquiry. This M00176-1 anti-p38 MAPK Rabbit Monoclonal antibody is tested on jurkat cell lysate, hela cells. It is guaranteed to work for IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-p38 MAPK Rabbit Monoclonal antibody M00176-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-31
Question
I was wanting to use your anti-p38 MAPK Rabbit Monoclonal antibody for IHC for human platelet on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human platelet identification?
E. Li
Verified customer
Asked: 2015-12-01
Answer
It shows on the product datasheet, M00176-1 anti-p38 MAPK Rabbit Monoclonal antibody has been validated for IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-12-01
Question
We have seen staining in human cervix carcinoma. Are there any suggestions? Is anti-p38 MAPK Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
D. Jones
Verified customer
Asked: 2013-10-10
Answer
According to literature cervix carcinoma does express MAPK14. According to Uniprot.org, MAPK14 is expressed in blood, peripheral blood, liver, b-cell, renal cell carcinoma, placenta, platelet, hepatoma, embryonic kidney, cervix carcinoma, leukemic t-cell, t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MAPK14 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 10727080
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Hepatoma, Pubmed ID: 10943842
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 7696354, 24275569
Peripheral blood, Pubmed ID: 7997261
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801, 18088087
Renal cell carcinoma, Pubmed ID: 11866441
T-cell, Pubmed ID: 21586573
Boster Scientific Support
Answered: 2013-10-10
