Product Info Summary
SKU: | A02999 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Goat |
Application: | ELISA, WB |
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Product info
Product Name
Anti-p33 ING1 Antibody
SKU/Catalog Number
A02999
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-p33 ING1 Antibody (Catalog # A02999). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-p33 ING1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02999)
Host
Goat
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human p33 ING1 protein (Inhibitor of growth family, member 1).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02999 is reactive to ING1 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
46738 MW
Background of ING1
p33 ING1 is a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53-signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. The accession number listed below is for variant (4) that encodes the longest isoform (D). Other synonyms for p33 ING1 include p33, p47, p33ING1, p24ING1c, p33ING1b, p47ING1a, growth inhibitor ING1, inhibitor of growth 1, tumor suppressor ING1 and growth inhibitory protein ING1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02999 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:4,000 - 1:14,000
WB: 1:500 - 1:3,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of p33 ING1 expression in purified recombinant GST tagged human ING1b (lane 1) and ING1c protein (lane 2). p33 ING1 was detected using goat anti-p33 ING1 Antigen Affinity purified polyclonal antibody (Catalog # A02999) at 1:1000. Personal communication Xiaolan Feng, U. Calgary.
Protein Target Info & Infographic
Gene/Protein Information For ING1 (Source: Uniprot.org, NCBI)
Gene Name
ING1
Full Name
Inhibitor of growth protein 1
Weight
46738 MW
Superfamily
ING family
Alternative Names
p33 ING1, p33ING1, ING-1, ING 1, Growth inhibitory protein ING1, Homo sapiens growth inhibitor p33ING1 (ING1) mRNA, complete cds, ING 1, Inhibitor of growth 1, Inhibitor of growth family member 1 ING1 p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a inhibitor of growth family member 1 inhibitor of growth protein 1|growth inhibitor ING1|growth inhibitory protein ING1|tumor suppressor ING1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ING1, check out the ING1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ING1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p33 ING1 Antibody (A02999)
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3 Customer Q&As for Anti-p33 ING1 Antibody
Question
We need to test anti-p33 ING1 antibody A02999 on human decidua for research purposes, then I may be interested in using anti-p33 ING1 antibody A02999 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
The products we sell, including anti-p33 ING1 antibody A02999, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-29
Question
I was wanting to use your anti-p33 ING1 antibody for WB for human decidua on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human decidua identification?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
As indicated on the product datasheet, A02999 anti-p33 ING1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human decidua in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-03
Question
I see that the anti-p33 ING1 antibody A02999 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-28