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Product Info Summary
| SKU: | M10087-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-P2Y6 Rabbit Monoclonal Antibody
View all P2Y6/P2RY6 Antibodies
SKU/Catalog Number
M10087-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-P2Y6 Rabbit Monoclonal Antibody catalog # M10087-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P2Y6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10087-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
19P56
Isotype
IgG
Immunogen
A synthesized peptide derived from human P2Y6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M10087-1 is reactive to P2RY6 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
36 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M10087-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human A549 whole cell, human THP-1 whole cell, human Caco-2 whole cell, human MCF-7 whole cell
IHC: human colon cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of P2Y6 using anti-P2Y6 antibody (M10087-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2Y6 antigen affinity purified monoclonal antibody (Catalog # M10087-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2Y6 at approximately 40 kDa. The expected band size for P2Y6 is at 36 kDa.
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Figure 2. IHC analysis of P2Y6 using anti-P2Y6 antibody (M10087-1).
P2Y6 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P2Y6 Antibody (M10087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For P2RY6 (Source: Uniprot.org, NCBI)
Gene Name
P2RY6
Full Name
P2Y purinoceptor 6
Weight
36 kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Cell division cycle-associated protein 3;Gene-rich cluster protein C8;Trigger of mitotic entry protein 1;TOME-1;CDCA3;C8, GRCC8, TOME1; P2RY6 P2Y6 pyrimidinergic receptor P2Y6 P2Y purinoceptor 6|G-coupled nucleotide receptor|P2 purinoceptor|P2Y6 receptor|pyrimidinergic receptor P2Y, G-protein coupled, 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on P2RY6, check out the P2RY6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for P2RY6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-P2Y6 Rabbit Monoclonal Antibody (M10087-1)
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