P2Y purinoceptor 6 Antibodies

P2Y purinoceptor 6 (P2RY6)

Receptor for extracellular UDP > UTP > ATP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.

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Gene Information

Human
Gene Name:	P2RY6
Uniprot:	Q15077
Entrez:	5031

Family

Belongs to:
G-protein coupled receptor 1 family

Alternative Names

G-coupled nucleotide receptor; MGC15335; P2 purinoceptor; P2RY6; P2Y6 receptor; P2Y6; P2Y6P2Y purinoceptor 6; pyrimidinergic receptor P2Y, G-protein coupled, 6

Molecular Weight

Mass (kDA):
36.429 kDA

Genomic Context

Human
Location:	11q13.4
Sequence:	11; NC_000011.10 (73264503..73298625)

Subcellular Localizations

Cell membrane; Multi-pass membrane protein.

Diseases

• Inflammation
• Radiation Injury
• Autoimmunity
• Cell Transformation, Neoplastic
• Respiratory Hypersensitivity
• Nerve Degeneration
• Inflammatory Bowel Diseases
• Bone Neoplasms
• Tissue Adhesions
• Intestinal Diseases
• Pneumonia
• Ulcerative Colitis

• Ulcer
• Osteosarcoma
• Malignant Neoplasms
• Neoplasms
• Cerebrovascular Accident
• Crohn Disease
• Colitis

Interacting Proteins

• CSE1L
• KPNB1
• KRTAP10-7
• PLSCR1

Pathways

• Cell Adhesion
• Cell Activation
• T Cell Activation
• Localization

PTMs

• Phosphorylation

Research Areas

• Apoptosis
• Cytokine Research
• GPCR
• Plasma Membrane Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/P2RY6

Best Uses Of The P2RY6 Marker

Boster Bio is the company to call if you need a reliable, highly specific P2Y6 anti-body. Boster Bio can provide antibodies for this marker. It also works to validate them across different platforms, with known positive and non-positive samples. The antibodies are known for their high affinity and specificity, and Boster rewards early reviewers with product credits. Boster rewards scientists all over the globe.

Anti-P2Y6/P2RY6 Signer

One of the high-specificity proteins is the P2Y6/P2RY6 protein marker. It is able to recognize specific antigens on other cellular surfaces. Three commercial antibodies that are meant to detect P2Y6 have failed, so investigators need to insist on strict control. Similar staining experiments with KO cell lines or heterologous expressions of antigen in cells are the best way to demonstrate specificity.

This antibody is a part of the Picoband(tm) catalog. The Boster Bio Anti-2Y6/P2RY6 Marker reacts with Human. This antibody can be stored at -20°C for upto one year, or at 4°C for upto a month. It has been used in IP, WB and ELISA tests.

This antibody was produced by removing a P2Y6 exon from a mice C57Bl/6J gene. In addition to KO mice, three commercially available antibodies gave clean bands on Western blots. Two of them immunostained bladder smooth muscles cells and one stained adjacent interstitial cell cells. However, attempts to validate the antibodies' specificity using P2Y6-knockout mice were unsuccessful. The protein levels in both groups were comparable on Western blots, immunostaining in wild type and knockout mice.

AMs are P2Y6-regulated IL-12p40. However, they cannot protect the immune system from Df-induced T2I. The P2Y6 receptor plays a crucial role in controlling immune responses in the respiratory tract. Your body may not be able to detect this protein. This could indicate a condition that can affect the functioning of your respiratory system.

Boster Bio's proprietary P2Y6/P2RY6 markings are based off data from the gene P2Y6/P2RY6. The gene is found on hematopoietic cells and is important for their protection against Df-induced allergic inflammation. Moreover, P2Y6/P2RY6 is involved in the cellular response to the P2Y6 protein.

The P2Y6/P2RY6 markers in mice that carry the gene inhibit the eosinophilic reaction. Restoring this protein reverses eosinophilic-lung inflammation in P2ry6fl/fl/Cre/+ mouse models. This gene is also expressed in the BAL fluids of P2Y6-deficient mouse.

Validation

The P2RY6 marker, a novel genetic marker, is used in lung carcinoma research. This gene is vital for controlling the influx of protective IFN g-producing NK cell. Lung inflammation is increased when this gene is deleted. Therefore, identifying patients with P2Y6-deficient cells is critical for the development of treatments for lung cancer. This marker has several applications, including the detection of pulmonary tumors and cancer stem cells.

The P2Y6 marker belongs to the Purinergic Receptor (P2R) subfamily, which regulates a wide range of physiological responses in the body. P2Rs play a variety of roles in the body, including placental function as well as innate immune defense. P2Y6 has been identified in various tissues, including the embryo, cervix, liver/spleenspleen, kidney, lung, and lungs.

Mice expressing the P2RY6 gene showed greater IL-12p40 levels than CCR2+ inflammatory monocytes in BAL fluid. CCR2+-inflammatory monocytes infiltrated the lungs even after the second dose Df. These cells also expressed IL-33, Clca3, and this indicates that the P2RY6 gene may be essential for the detection lung cancer.

P2Y6 receptor not only regulates IL-12, but also plays a key role in early alveolar IL-12 formation. This gene can be found on the surface cells of immune cells including T cells. However, mice can be genetically modified to have lower serum levels of IL-12. P2Y6 receptor was associated with several inflammatory diseases, such as lung cancer. It is important not to forget that these markers may not be sensitive to all Df-induced immune replies.

Applications

The P2RY6 gene encodes a member of the Purinergic Receptor subfamily. It activates using UDP and not ATP. Its function is still unknown, but it is thought to be involved in placental function and innate immune defense. It has been discovered in many tissues including the cervix (embryo), liver/spleen, as well as placenta.

Despite the negative effects of microglia on neurodegenerative disease, they are an important source for immunity and maintain CNS homeostasis. Researchers are now using P2RY12-CreER mice specifically to label microglia and other brain macrophages. Jackson Labs plans to soon release P2RY12–CreER mice. Once they are created, these mice will allow researchers to study how cells respond to brain injury.

The P2RY6 marker can also be used to detect MMP-9 levels in IEC. After FGF2 stimulation, MMP-9 gene activity was higher in IECs of mice P2RY6/P2RY6. These data suggest that P2Y6 receptors regulate the activity of multiple receptors at the surface of IEC. The P2RY6 receptor has many applications.

P2RY6 also has been implicated recently in neurodegenerative disease. It also regulates interleukin-8 production by macrophages and monocytes. Therefore, targeting P2RY6 may provide a new approach to treating diseases related to microglia activation. Its cell involvement in neurodegenerative or inflammatory diseases is an important aspect of its importance.

Availability

A patient's blood can be tested for the presence of the P2RY6 gene. This marker is useful in diagnosing inflammatory conditions. The P2RY6 receptor is a member of the family of G protein-coupled receptors (GPCRs) that has several subtypes with variable pharmacological selectivity and overlap with adenosine nucleotides. P2RY6 is sensitive to UDP and UTP but not to ATP. The P2RY6 receptor signaling pathway might be a potential therapeutic target to treat monosodium urinary-associated inflammatory disease.

A variety of chemokine receptive molecules on the IEC regulate the expression level of the P2RY6 Gene. It regulates the release of these chemokines, and maintains homeostasis at the IEC. It has been shown that FGF2 response can be affected by the presence of the P2RY6 marker within cells.

The adenosine A2A receptor gene is highly expressed. However, the P2RY6/ IECs show a slightly lower gene expression than WT cells. There was no significant difference between P2RY6// IECs and WT mice in this respect, which suggests that it is linked to the development PD. Although P2RY6 wasn't detected in the control mice it was detected in other cells. This suggests that it plays a role in inflammatory brain diseases such as Parkinson's disease.