Anti-p29 ING4 Antibody

Product Name

Anti-p29 ING4 Antibody

View all Ing4 Antibodies

SKU/Catalog Number

A02664

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-p29 ING4 Antibody (Catalog # A02664). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-p29 ING4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02664)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human p29 ING4 protein (Inhibitor of growth family, member 4). This sequence only shows homology to isoform 1 for ING4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02664 is reactive to ING4 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

28530 MW

Background of Ing4

p29 ING4 is a tumor suppressor protein similar to ING1 that may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. p29 ING4 has been shown to suppress brain tumor angio-genesis through transcriptional repression of RelA/ NFKB3 target genes when complexed with RelA. p29 ING4 may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. p29 ING4 shows a nuclear localization and interacts with EP300, TP53, RelA, inhibits cell growth, and induces apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) that encodes the longest isoform.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02664 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:3,000 - 1:12,000
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

Gene/Protein Information For ING4 (Source: Uniprot.org, NCBI)

Alternative Names

p29 ING4, p29ING4, ING 4, ING-4, Inhibitor of growth family member 4, Inhibitor of growth family member 4 long isoform, Inhibitor of growth protein 4, MGC12557 Ing4|D6Wsu147, D6Wsu147e, D6Xrf9, D6Xrf92, p29I, p29ING4|inhibitor of growth family, member 4|inhibitor of growth protein 4|ING1-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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