Anti-P15RS/RPRD1A Antibody Picoband™

Product Name

Anti-P15RS/RPRD1A Antibody Picoband™

View all P15RS Antibodies

SKU/Catalog Number

A10062-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-P15RS/RPRD1A Antibody Picoband™ catalog # A10062-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-P15RS/RPRD1A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10062-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human P15RS/RPRD1A recombinant protein (Position: E76-D235).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A10062-1 is reactive to RPRD1A in Human

Applications

A10062-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

41409 MW

Background of P15RS

This gene encodes a cell-cycle and transcription regulatory protein. The encoded protein interacts with the cell cycle inhibitor cyclin-dependent kinase 4 inhibitor B and may function as a negative regulator of G(1)/S phase progression. This protein also forms homo- and hetrodimers with the protein, regulation of nuclear pre-mRNA domain-containing protein 1B, to form a scaffold that interacts with the C-terminal domain of RNA polymerase II subunit B1 and regulates several aspects of transcription. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 16.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of P15RS/RPRD1A using anti-P15RS/RPRD1A antibody (A10062-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P15RS/RPRD1A antigen affinity purified polyclonal antibody (Catalog # A10062-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P15RS/RPRD1A at approximately 36 kDa. The expected band size for P15RS/RPRD1A is at 36 kDa.

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Figure 2. Flow Cytometry analysis of RT4 cells using anti-P15RS/RPRD1A antibody (A10062-1).
Overlay histogram showing RT4 cells stained with A10062-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P15RS/RPRD1A Antibody (A10062-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For RPRD1A (Source: Uniprot.org, NCBI)

Alternative Names

Cyclin-dependent kinase inhibitor 2B-related protein (p15INK4B-related protein); Cyclin-dependent kinase inhibitor 2B-related protein; FLJ10656; HsT3101; MGC19513; p15INK4B-related protein; P15RScyclin-dependent kinase 2B-inhibitor-related protein; regulation of nuclear pre-mRNA domain containing 1A; regulation of nuclear pre-mRNA domain-containing protein 1A RPRD1A HsT3101, P15RS regulation of nuclear pre-mRNA domain containing 1A regulation of nuclear pre-mRNA domain-containing protein 1A|CDKN2B-related protein|Cyclin-dependent kinase inhibitor 2B-related protein (p15INK4B-related protein)|cyclin-dependent kinase 2B-inhibitor-related protein|p15INK4B-related protein

For more info on RPRD1A, check out the RPRD1A Infographic

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In this infographic, you will see the following information for RPRD1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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