Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4)
|Ig Isotype||Mouse IgG1|
|Applications||IHC, ICC, WB|
|Product Name||Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for P-Glycoprotein (MDR), ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1060)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||Multidrug resistance protein 1A|
|Tissue Specificity||ABCB1: Expressed in liver, kidney, small intestineand brain.|
|Alternative Names||Multidrug resistance protein 1A;220.127.116.11;ATP-binding cassette sub-family B member 1A;MDR1A;Multidrug resistance protein 3;P-glycoprotein 3;Abcb1a;Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3;|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein.|
|Molecular Weight||140647 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. .|
*You can search these to find other products in these research areas.
|Background||P-Glycoprotein,, also known as Multidrug Resistance 1(MDR1), is one of the ATP-binding cassette transporters family. P-glycoprotein-1 is involved in the transport of 3 of these protease inhibitors in vitro. MDR1 gene is mapped to the 7q21.1 by in situ hybridization. The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to abcb1 antibody, mdr1 antibody, p gp antibody