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Product Info Summary
| SKU: | M00634-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Osteopontin (SPP1) Mouse Monoclonal Antibody [Clone ID: OTI5E4]
View all Osteopontin/OPN Antibodies
SKU/Catalog Number
M00634-1
Size
100 µl
Description
Boster Bio SPP1 mouse monoclonal antibody, clone OTI5E4 (formerly 5E4). Catalog# M00634-1. Tested in IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Osteopontin (SPP1) Mouse Monoclonal Antibody [Clone ID: OTI5E4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00634-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5E4
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 17-314 of human SPP1 (NP_001035147) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00634-1 is reactive to SPP1 in Human
Applications
M00634-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Protein Target Info & Infographic
Gene/Protein Information For SPP1 (Source: Uniprot.org, NCBI)
Gene Name
SPP1
Full Name
Osteopontin
Weight
Superfamily
osteopontin family
Alternative Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin SPP1 BNSP, BSPI, ETA-1, OPN secreted phosphoprotein 1 osteopontin|SPP1/CALPHA1 fusion|early T-lymphocyte activation 1|nephropontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)|secreted phosphoprotein 1 variant 6|urinary stone protein|uropontin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPP1, check out the SPP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Osteopontin (SPP1) Mouse Monoclonal Antibody [Clone ID: OTI5E4] (M00634-1)
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