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Product Info Summary
| SKU: | A00474 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-OGT Antibody
View all O-GlcNAc Transferase p110 subunit Antibodies
SKU/Catalog Number
A00474
Size
100ul
Form
Liquid
Description
Boster Bio Anti-OGT Antibody catalog # A00474. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-OGT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00474)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 857-1036 of human OGT (NP_858059.1).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00474 is reactive to OGT in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
116925 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00474 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of OGT using Anti-OGT Antibody (A00474).
ICC staining OGT in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-OGT Antibody A00474
Western blot analysis of OGT on Hela (1) and SiHa (2) cell lysate using anti-OGT antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For OGT (Source: Uniprot.org, NCBI)
Gene Name
OGT
Full Name
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
Weight
116925 MW
Superfamily
glycosyltransferase 41 family
Alternative Names
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit;2.4.1.255 OGT HINCUT-1, HRNT1, MRX106, O-GLCNAC1, OGT O-linked N-acetylglucosamine (GlcNAc) transferase UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit|O-GlcNAc transferase p110 subunit|O-GlcNAc transferase subunit p110|O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)|O-linked N-acetylglucosamine transferase 110 kDa subunit|UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase|uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OGT, check out the OGT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OGT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OGT Antibody (A00474)
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